Artigo
Highly Sensitive Electrochemical Sensor for Determination of Vitamin D in Mixtures of Water-Ethanol
Registro en:
Electroanalysis. Weinheim: Wiley-v C H Verlag Gmbh, v. 26, n. 12, p. 2783-2788, 2014.
1040-0397
10.1002/elan.201400451
WOS:000345978300029
Autor
Cincotto, Fernando H. [UNESP]
Canevari, Thiago C. [UNESP]
Machado, Sergio A. S. [UNESP]
Resumen
This paper describes the electrochemical determination of vitamin D-2 (ergocalciferol) and D-3 (cholecalciferol) in mixed organic/water solvent, using a glassy carbon electrode (GCE). The mixing ratio of organic/water solvent has an important influence on the electrocatalytic response of D vitamins on the surface of the glassy carbon electrode. Well-defined peaks for Vitamin D-2 and D-3 were observed in a 40% ethanol/60% water solution with lithium perchlorate as the support electrolyte. This study demonstrated that the glassy carbon electrode is highly sensitive for the determination of vitamin D-2 and D-3, with a limit of detection of 0.13 and 0.118 mu molL(-1), respectively. No significant interference was seen for vitamins A, E and K in the detection of vitamin D. Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) State Univ Sao Paulo, Inst Chem, BR-13560970 Sao Carlos, SP, Brazil State Univ Sao Paulo, Inst Chem, BR-13560970 Sao Carlos, SP, Brazil FAPESP: 11/23047-7 FAPESP: 12/18339-1