Artigo
Inflammatory response of endothelial cells to hepatitis C virus recombinant envelope glycoprotein 2 protein exposure
Registro en:
Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 109, n. 6, p. 748-756, 2014.
0074-0276
10.1590/0074-0276140090
S0074-02762014000600748
S0074-02762014000600748.pdf
4066413997908572
6720223715917381
0000-0002-3350-8308
Autor
Urbaczek, Ana Carolina
Ribeiro, Lívia Carolina De Abreu
Ximenes, Valdecir Farias [UNESP]
Afonso, Ana
Nogueira, Camila Tita
Generoso, Wesley Cardoso
Alberice, Juliana Vieira
Rudnicki, Martina
Ferrer, Renila
Fonseca, Luiz Marcos Da
Costa, Paulo Inácio Da
Resumen
The hepatitis C virus (HCV) encodes approximately 10 different structural and non-structural proteins, including the envelope glycoprotein 2 (E2). HCV proteins, especially the envelope proteins, bind to cell receptors and can damage tissues. Endothelial inflammation is the most important determinant of fibrosis progression and, consequently, cirrhosis. The aim of this study was to evaluate and compare the inflammatory response of endothelial cells to two recombinant forms of the HCV E2 protein produced in different expression systems (Escherichia coli and Pichia pastoris). We observed the induction of cell death and the production of nitric oxide, hydrogen peroxide, interleukin-8 and vascular endothelial growth factor A in human umbilical vein endothelial cells (HUVECs) stimulated by the two recombinant E2 proteins. The E2-induced apoptosis of HUVECs was confirmed using the molecular marker PARP. The apoptosis rescue observed when the antioxidant N-acetylcysteine was used suggests that reactive oxygen species are involved in E2-induced apoptosis. We propose that these proteins are involved in the chronic inflammation caused by HCV. Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Escola de Ciências Farmacêuticas Departamento de Análises Clínicas Laboratório de Imunologia Clínica Universidade Estadual Paulista Julio de Mesquita Filho Faculdade de Ciências Departamento de Química Universidade Nova de Lisboa Instituto de Higiene e Medicina Tropcal Departamento de Parasitologia Médica Universidade Federal de São Carlos Departamento de Morfologia e Patologia Universidade Federal de São Carlos Departamento de Genética e Evolução Universidade de São Paulo Instituto de Química de São Carlos Departamento de Química e Física Molecular Universidade de São Paulo Escola de Ciências Farmacêuticas Universidade Estadual Paulista Julio de Mesquita Filho Faculdade de Ciências Departamento de Química