The effects of copper ions on the synthesis of periplasmic and membrane proteins in Acidithiobaeillus ferrooxidans as analyzed by SDS-PAGE and 2D-PAGE

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorFelicio, A. P.
dc.creatorGarcia, O.
dc.creatorBertolini, Maria Celia
dc.creatorOttoboni, LMM
dc.creatorNovo, MTM
dc.date2014-05-20T15:20:44Z
dc.date2016-10-25T17:53:55Z
dc.date2014-05-20T15:20:44Z
dc.date2016-10-25T17:53:55Z
dc.date2003-10-01
dc.date.accessioned2017-04-05T23:27:23Z
dc.date.available2017-04-05T23:27:23Z
dc.identifierHydrometallurgy. Amsterdam: Elsevier B.V., v. 71, n. 1-2, p. 165-171, 2003.
dc.identifier0304-386X
dc.identifierhttp://hdl.handle.net/11449/31970
dc.identifierhttp://acervodigital.unesp.br/handle/11449/31970
dc.identifier10.1016/S0304-386X(03)00153-1
dc.identifierWOS:000186357900021
dc.identifierhttp://dx.doi.org/10.1016/S0304-386X(03)00153-1
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/876406
dc.descriptionThe effects of 200 mM copper ions on the synthesis of membrane and periplasmic proteins were investigated in iron-grown cells of Acidithiobacillus ferrooxidans (At. ferrooxidans). Total membrane protein profiles of cells grown in the absence of copper ions (unadapted cells) and in the presence of copper ions (copper-adapted cells) were compared by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE). Crude preparations of outer membrane and periplasmic proteins were analyzed by SDS-PAGE. The synthesis of proteins was diminished or increased in the presence of copper ions. Low molecular weight proteins (< 14 kDa) were significantly repressed by copper. These proteins are probably acidic proteins located in the outer membrane. An over-expression of a periplasmic protein of about 17 kDa was detected in the copper-adapted cells and was assumed to be rusticyanin, a 16.5-kDa periplasmic copper protein present in At. ferrooxidans cells and involved in the electron-transport chain of the iron oxidation pathway. To our knowledge, this is the first report of a possible involvement of the rusticyanin and outer membrane proteins in the mechanism of copper resistance in At. ferrooxidans. (C) 2003 Elsevier B.V. All rights reserved.
dc.languageeng
dc.publisherElsevier B.V.
dc.relationHydrometallurgy
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectcopper resistance
dc.subjectouter membrane protein
dc.subjectperiplasmic protein
dc.subjectAcidithiobacillus ferrooxidans
dc.subject2D-PAGE
dc.subjectmetal resistance
dc.subjectrusticyanin
dc.titleThe effects of copper ions on the synthesis of periplasmic and membrane proteins in Acidithiobaeillus ferrooxidans as analyzed by SDS-PAGE and 2D-PAGE
dc.typeOtro


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