Artigo
Assessment of experimental infection for dogs using Gallus gallus chorioallantoic membranes inoculated with Neospora caninum
Registro en:
Revista Brasileira de Parasitologia Veterinaria. São Paulo: Brazilian Coll Veterinary Parasitology, v. 22, n. 4, p. 565-570, 2013.
1984-2961
S1984-29612013000400018
WOS:000330849100018
S1984-29612013000400018.pdf
3254990612451836
Autor
Munhoz, Alexandre Dias
Patriarca Mineo, Tiago Wilson
Alessi, Antonio Carlos [UNESP]
Gomes Lopes, Carlos Wilson
Machado, Rosangela Zacarias [UNESP]
Resumen
The aim of this study was to evaluate parasitism kinetics and tissue lesions in the first week of infection by Neospora caninum in dogs fed Gallus gallus chorioallantoic membranes (CMs) previously infected in ovo. Five two-month-old pups were used. Each dog was given five CMs that were previously infected with N. caninum via the oral route. Four animals were euthanized in the first week of infection. All four dogs had their stools examined one week prior to and up to the day they were euthanized. The stools of the uneuthanized dog were collected for 30 days. After euthanasia, organ sections were utilized for histopathology, immunohistochemistry, indirect immunofluorescent tissue reactions, PCR and real-time PCR to detect parasites. Necropsy revealed that the small and large intestines, spleen, and lungs were affected. No oocysts or N. caninum DNA were identified in the stool samples. Real-time PCR was the most sensitive technique used to detect the protozoa in tissues, which were identified in 41% of the analyzed samples. Our results indicate that an experimental model using previously infected CMs appears to be a useful model for the study of the host-parasite relationship during the infection's acute phase. Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Programa Nacional de Cooperacao Academica - Novas Fronteiras Univ Estadual Santa Cruz UESC, Dept Ciencias Agra & Ambientais, Ilheus, BA, Brazil Univ Fed Uberlandia UFU, Inst Ciencias Biomed, Uberlandia, MG, Brazil Univ Estadual Julio Mesquita Filho UNESP, Dept Patol Vet, Jaboticabal, SP, Brazil Univ Fed Rural Rio de Janeiro, Dept Parasitol Anim, Seropedica, RJ, Brazil Univ Estadual Julio Mesquita Filho UNESP, Dept Patol Vet, Jaboticabal, SP, Brazil Programa Nacional de Cooperacao Academica - Novas Fronteiras1512/2007-NF/CAPES