Artigo
Structure of the polypeptide crotamine from the Brazilian rattlesnake Crotalus durissus terrificus
Registro en:
Acta Crystallographica Section D-biological Crystallography. Hoboken: Wiley-blackwell, v. 69, p. 1958-1964, 2013.
0907-4449
10.1107/S0907444913018003
WOS:000325403900010
9162508978945887
0000-0003-2460-1145
Autor
Coronado, Monika A. [UNESP]
Gabdulkhakov, Azat
Georgieva, Dessislava
Sankaran, Banumathi
Murakami, Mario T.
Arni, Raghuvir K. [UNESP]
Betzel, Christian
Resumen
The crystal structure of the myotoxic, cell-penetrating, basic polypeptide crotamine isolated from the venom of Crotalus durissus terrificus has been determined by single-wavelength anomalous dispersion techniques and refined at 1.7 angstrom resolution. The structure reveals distinct cationic and hydrophobic surface regions that are located on opposite sides of the molecule. This surface-charge distribution indicates its possible mode of interaction with negatively charged phospholipids and other molecular targets to account for its diverse pharmacological activities. Although the sequence identity between crotamine and human beta-defensins is low, the three-dimensional structures of these functionally related peptides are similar. Since crotamine is a leading member of a large family of myotoxic peptides, its structure will provide a basis for the design of novel cell-penetrating molecules. Deutsche Forschungsgemeinschaft Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) DAAD Alexander von Humboldt Foundation (AvH), Bonn, Germany National Institutes of Health, National Institute of General Medical Sciences Howard Hughes Medical Institute US Department of Energy Sao Paulo State Univ, UNESP IBILCE, Dept Phys, Multi User Ctr Biomol Innovat, BR-15054000 Sao Jose Do Rio Preto, SP, Brazil Univ Hamburg, Inst Biochem & Mol Biol, D-20146 Hamburg, Germany RAS, Inst Prot Res, Pushchino 142290, Moscow Region, Russia Lawrence Berkeley Natl Lab, Phys Biosci Div, Berkeley, CA 94702 USA Natl Ctr Energy & Mat Res, Biosci Natl Lab, BR-13083970 Campinas, SP, Brazil Sao Paulo State Univ, UNESP IBILCE, Dept Phys, Multi User Ctr Biomol Innovat, BR-15054000 Sao Jose Do Rio Preto, SP, Brazil Deutsche ForschungsgemeinschaftBE 1443-18-1 Deutsche Forschungsgemeinschaft26-1 DAADPROBAL 50754442 CAPES: NR-8248/12-5 US Department of EnergyDE-AC02-05CH11231