Artigo
Production of the First Effective Hyperimmune Equine Serum Antivenom against Africanized Bees
Registro en:
Plos One. San Francisco: Public Library Science, v. 8, n. 11, 10 p., 2013.
1932-6203
10.1371/journal.pone.0079971
WOS:000327254700167
WOS000327254700167.pdf
7538556085505819
2901888624506535
0000-0002-1650-257X
Autor
Santos, Keity Souza
Stephano, Marco Antonio
Marcelino, Jose Roberto
Resende Ferreira, Virginia Maria
Rocha, Thalita
Caricati, Celso
Higashi, Hisako Gondo
Moro, Ana Maria
Kalil, Jorge Elias
Malaspina, Osmar [UNESP]
Morato Castro, Fabio Fernandes
Palma, Mario Sergio [UNESP]
Resumen
Victims of massive bee attacks become extremely ill, presenting symptoms ranging from dizziness and headache to acute renal failure and multiple organ failure that can lead to death. Previous attempts to develop specific antivenom to treat these victims have been unsuccessful. We herein report a F(ab)'(2)-based antivenom raised in horse as a potential new treatment for victims of multiple bee stings. The final product contains high specific IgG titers and is effective in neutralizing toxic effects, such as hemolysis, cytotoxicity and myotoxicity. The assessment of neutralization was revised and hemolysis, the primary toxic effect of these stings, was fully neutralized in vivo for the first time. Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) FINEP (CT-BIOTEC-Anticorpos -MCTFINEP) Butantan Institute Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Univ Sao Paulo, Sch Med, FMUSP, Div Clin Immunol & Allergy, Sao Paulo, Brazil Inst Invest Immunol INCT, Sao Paulo, Brazil Univ Sao Paulo, Fac Ciencias Farmaceut, Sao Paulo, Brazil Butantan Inst, Div Hyperimmune Plasmas, Sao Paulo, Brazil Univ Sao Francisco, Sch Med, BR-12900000 Braganca Paulista, SP, Brazil Butantan Inst, Special Pilot Lab Res & Dev Vet Immunobiol, Sao Paulo, Brazil Inst Technol Parana, Curitiba, Parana, Brazil Butantan Inst, Lab Biopharmaceut Anim Cells, Sao Paulo, Brazil Univ Sao Paulo, Sch Med, Heart Inst InCor, LIM 19, Sao Paulo, Brazil Univ Sao Paulo State UNESP, Ctr Study Social Insects, Inst Biosci, Dept Biol, Rio Claro, SP, Brazil Univ Sao Paulo State UNESP, Ctr Study Social Insects, Inst Biosci, Dept Biol, Rio Claro, SP, Brazil FAPESP: 11/51684-1