Diabetes mellitus activates fetal gene program and intensifies cardiac remodeling and oxidative stress in aged spontaneously hypertensive rats

dc.contributorUniversidade Estadual Paulista (Unesp)
dc.creatorRosa, Camila Moreno [UNESP]
dc.creatorXavier, Natasha Priscilla [UNESP]
dc.creatorCampos, Dijon Henrique [UNESP]
dc.creatorFernandes, Ana Angelica Henrique [UNESP]
dc.creatorMariano Cezar, Marcelo Diarcadia [UNESP]
dc.creatorMartinez, Paula Felippe [UNESP]
dc.creatorCicogna, Antonio Carlos [UNESP]
dc.creatorGimenes, Camila [UNESP]
dc.creatorGimenes, Rodrigo [UNESP]
dc.creatorOkoshi, Marina Politi [UNESP]
dc.creatorOkoshi, Katashi [UNESP]
dc.date2014-12-03T13:10:30Z
dc.date2014-12-03T13:10:30Z
dc.date2013-10-17
dc.date.accessioned2023-09-09T09:55:32Z
dc.date.available2023-09-09T09:55:32Z
dc.identifierhttp://dx.doi.org/10.1186/1475-2840-12-152
dc.identifierCardiovascular Diabetology. London: Biomed Central Ltd, v. 12, 9 p., 2013.
dc.identifier1475-2840
dc.identifierhttp://hdl.handle.net/11449/112201
dc.identifier10.1186/1475-2840-12-152
dc.identifierWOS:000327610800004
dc.identifierWOS000327610800004.pdf
dc.identifier9418970103564137
dc.identifier4463138671998432
dc.identifier1590971576309420
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8761833
dc.descriptionBackground: The combination of systemic arterial hypertension and diabetes mellitus (DM) induces greater cardiac remodeling than either condition alone. However, this association has been poorly addressed in senescent rats. Therefore, this study aimed to analyze the influence of streptozotocin-induced DM on ventricular remodeling and oxidative stress in aged spontaneously hypertensive rats (SHR).Methods: Fifty 18 month old male SHR were divided into two groups: control (SHR, n = 25) and diabetic (SHR-DM, n = 25). DM was induced by streptozotocin (40 mg/kg, i.p.). After nine weeks, the rats underwent echocardiography and myocardial functional study in left ventricular (LV) isolated papillary muscle preparations. LV samples were obtained to measure myocyte diameters, interstitial collagen fraction, and hydroxyproline concentration. Gene expression of atrial natriuretic peptide (ANP) and alpha- and beta-myosin heavy chain (MyHC) isoforms was evaluated by RT-PCR. Serum oxidative stress was assessed by measuring lipid hydroperoxide concentration and superoxide dismutase and glutathione peroxidase activities. Statistics: Student's t test or Mann-Whitney test, p < 0.05.Results: SHR-DM presented higher blood glucose (487 +/- 29 vs. 89.1 +/- 21.1 mg/dL) and lower body weight (277 +/- 26 vs. 339 +/- 38 g). Systolic blood pressure did not differ between groups. Echocardiography showed LV and left atrial dilation, LV diastolic and relative wall thickness decrease, and LV systolic and diastolic function impairment in SHR-DM. Papillary muscle study showed decreased myocardial contractility and contractile reserve in SHR-DM. Myocyte diameters and myocardial interstitial collagen fraction and hydroxyproline concentration did not differ between groups. Increased serum pro-oxidant activity and gene expression of ANP and beta/alpha-MyHC ratio were observed in DM.Conclusion: Diabetes mellitus induces cardiac dilation and functional impairment, increases oxidative stress and activates fetal gene program in aged spontaneously hypertensive rats.
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionSao Paulo State Univ, UNESP, Botucatu Med Sch, Dept Internal Med, Botucatu, SP, Brazil
dc.descriptionSao Paulo State Univ, UNESP, Inst Biosci, Dept Chem & Biochem, Botucatu, SP, Brazil
dc.descriptionUNESP, Fac Med Botucatu, Dept Clin Med, BR-18618970 Botucatu, SP, Brazil
dc.descriptionSao Paulo State Univ, UNESP, Botucatu Med Sch, Dept Internal Med, Botucatu, SP, Brazil
dc.descriptionSao Paulo State Univ, UNESP, Inst Biosci, Dept Chem & Biochem, Botucatu, SP, Brazil
dc.descriptionUNESP, Fac Med Botucatu, Dept Clin Med, BR-18618970 Botucatu, SP, Brazil
dc.descriptionCNPq: 306845/2012-1
dc.descriptionCNPq: 306857/2012-0
dc.descriptionFAPESP: 09/54506-7
dc.format9
dc.languageeng
dc.publisherBiomed Central Ltd.
dc.relationCardiovascular Diabetology
dc.relation5.235
dc.relation2,157
dc.rightsAcesso aberto
dc.sourceWeb of Science
dc.subjectDiabetes mellitus
dc.subjectOxidative stress
dc.subjectSystemic hypertension
dc.subjectSpontaneously hypertensive rats
dc.subjectElderly
dc.titleDiabetes mellitus activates fetal gene program and intensifies cardiac remodeling and oxidative stress in aged spontaneously hypertensive rats
dc.typeArtigo


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