Artigo
(S)-Goniothalamin induces DNA damage, apoptosis, and decrease in BIRC5 messenger RNA levels in NCI-H460 cells
Registro en:
Human & Experimental Toxicology. London: Sage Publications Ltd, v. 33, n. 1, p. 3-13, 2014.
0960-3271
10.1177/0960327113491506
WOS:000328598600001
Autor
Semprebon, S. C.
Fatima, A. de
Lepri, S. R.
Sartori, D.
Ribeiro, L. R. [UNESP]
Mantovani, M. S.
Resumen
(R)-Goniothalamin (R-GNT) is a secondary metabolite isolated from the plants of the genus Goniothalamus. This molecule has attracted the attention of researchers because of its selective cytotoxicity against tumor cells and its ability to induce apoptosis. (S)-Goniothalamin (S-GNT) is a synthetic enantiomer of R-GNT, and its mechanism of action is largely unknown. In this study, we investigated the activity of S-GNT in a human non-small cell lung cancer NCI-H460 cells. We observed that the cells exposed to this compound exhibited cytotoxicity in a concentration-dependent manner. Based on the data obtained through the assessment of apoptosis induction in situ and the comet assay, we suggest that this cytotoxicity occurs due to the potential ability of this molecule to induce DNA damage with the consequent induction of cell death via apoptosis. A significant reduction in the messenger RNA levels of baculoviral inhibitor of apoptosis repeat-containing 5 (BIRC5) gene that encodes the survivin protein was found. This novel finding may explain the inhibition of cell proliferation and induction of apoptosis in tumor cells caused by this compound. Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Fundacao Araucaria Univ Estadual Londrina, Dept Biol Geral, BR-86051990 Londrina, Parana, Brazil Univ Fed Minas Gerais, Dept Quim, Belo Horizonte, MG, Brazil Univ Estadual Paulista, Dept Patol, Sao Paulo, Brazil Univ Estadual Paulista, Dept Patol, Sao Paulo, Brazil