Avaliação da segurança e da atividade imunomoduladora dos extratos de Uncaria tomenosa e Uncaria guianensis sobre a patogenia dos Diabetes Melito autoimune induzido pela estreptozotocina

dc.contributorViero, Rosa Marlene [UNESP]
dc.contributorSartori, Alexandrina [UNESP]
dc.contributorUniversidade Estadual Paulista (Unesp)
dc.creatorDomingues, Alexandre [UNESP]
dc.date2014-06-11T19:33:24Z
dc.date2014-06-11T19:33:24Z
dc.date2011-02-25
dc.date2011
dc.date.accessioned2023-09-09T07:54:27Z
dc.date.available2023-09-09T07:54:27Z
dc.identifierDOMINGUES, Alexandre. Avaliação da segurança e da atividade imunomoduladora dos extratos de Uncaria tomenosa e Uncaria guianensis sobre a patogenia dos Diabetes Melito autoimune induzido pela estreptozotocina. 2011. 159 f. Tese (doutorado) - Universidade Estadual Paulista, Faculdade de Medicina de Botucatu, 2011.
dc.identifierhttp://hdl.handle.net/11449/104553
dc.identifier000638839
dc.identifierdomingues_a_dr_botfm.pdf
dc.identifier33004064056P5
dc.identifier1453564171333848
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8754642
dc.descriptionUncaria tomentosa (Willd.) DC (Rubiaceae) is a large woody vine that is native to the Amazon and Central American rainforests and is widely used in traditional medicine for its immunomodulatory and anti-inflammatory activities. The present work used in vivo immunotoxic and in vitro immunomodulatory experiments to investigate the effects of a pentacyclic oxindole alkaloid extract from U. tomentosa bark on lymphocyte phenotype, Th1/Th2 cytokine production, cellular proliferation and cytotoxicity. For the in vivo immunotoxicity testing, BALB/c male mice were treated once a day with 125, 500 or 1250 mg/kg of U. tomentosa extract for 28 days. For the in vitro protocol, lymphocytes were cultured with 10 to 500 μL/mL of the extract for 48 h. The extract increased the cellularity of splenic white pulp and the thymic medulla and increased the number of T helper lymphocytes and B lymphocytes. Also, a large stimulatory effect on lymphocyte viability was observed. However, mitogen-induced T lymphocyte proliferation was significantly inhibited at higher concentrations of U. tomentosa extract. Furthermore, an immunological polarization toward a Th2 cytokine profile was observed. These results suggest that the U. tomentosa aqueous-ethanol extract was not immunotoxic to mice and was able to modulate distinct patterns of the immune system in a dose-dependent manner
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.format159 f.
dc.languagepor
dc.publisherUniversidade Estadual Paulista (Unesp)
dc.rightsAcesso aberto
dc.rightsLOCKSS system has permission to collect, preserve, and serve this Archival Unit
dc.sourceAleph
dc.subjectDiabetes - Aspestos imunológicos
dc.subjectUncaria tomentosa
dc.subjectImmune system
dc.titleAvaliação da segurança e da atividade imunomoduladora dos extratos de Uncaria tomenosa e Uncaria guianensis sobre a patogenia dos Diabetes Melito autoimune induzido pela estreptozotocina
dc.typeTese de doutorado


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