Otro
Optimization of an amperometric biosensor for the detection of hepatitis C virus using fractional factorial designs
Registro en:
Journal of The Brazilian Chemical Society. São Paulo: Soc Brasileira Quimica, v. 19, n. 4, p. 782-787, 2008.
0103-5053
10.1590/S0103-50532008000400024
S0103-50532008000400024
WOS:000256957100023
Autor
Uliana, Carolina V.
Riccardi, Carla S.
Tognolli, Joao O.
Yamanaka, Hideko
Resumen
Fractional factorial design and factorial with center point design were applied to the development of an amperometric biosensor for the detection of the hepatitis C virus. Biomolecules were immobilized by adsorption on graphite electrodes modified with siloxane-poly(propyleneoxide) hybrid matrix prepared using the sol-gel method. Several parameters were optimized, such as the streptavidin concentration at 0.01 mg mL(-1) and 1.0% bovine serum albumin, the incubation time of the electrodes in the complementary DNA solution for 30 minutes and a 1: 1500 dilution of the avidin-peroxidase conjugate, among others. The application of chemometric studies has been efficient, since the best conditions have been established with a restricted number of experiments, indicating the influence of different factors on the system.