Otro
Síntese, caracterização e estudos de interação de um análogo da antitoxina CcdA empregando fluorescência no estado estacionário
Registro en:
Química Nova. Sociedade Brasileira de Química, v. 33, n. 4, p. 841-845, 2010.
0100-4042
10.1590/S0100-40422010000400014
S0100-40422010000400014
WOS:000278322500014
S0100-40422010000400014.pdf
Autor
Cotrim, Camila Aparecida
Garrido, Saulo Santesso
Trovatti, Eliane
Marchetto, Reinaldo
Resumen
Toxin-antitoxin (TA) systems contribute to plasmid stability by a mechanism called post-segregational killing. The ccd was the first TA system to be discovered with CcdB being the toxin and CcdA the antitoxin. CcdA, an 8.3 kDa protein, interacts with CcdB (11.7 kDa), preventing the cytotoxic activity of CcdB on the DNA gyrase. As an approach to understanding this interaction, CcdA41, a polypeptide derived from CcdA, was synthesized by solid-phase methodology and its interaction with CcdB was analyzed by steady state fluorescence. CcdA41 formed a stable complex with CcdBET2, a peptide based on CcdB, the more recently described bacterial topoisomerase inhibitor. Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)