Otro
Production, purification, crystallization and preliminary X-ray diffraction studies of the nucleoside diphosphate kinase b from Leishmania major
Registro en:
Acta Crystallographica Section F-structural Biology and Crystallization Communications. Malden: Wiley-blackwell Publishing, Inc, v. 65, p. 1116-1119, 2009.
1744-3091
10.1107/S1744309109037567
WOS:000271421800010
WOS000271421800010.pdf
Autor
Costa Tonoli, Celisa Caldana
Vieira, Plinio Salmazo
Ward, Richard John
Arni, Raghuvir Krishnaswamy
Cavalcante de Oliveira, Arthur Henrique
Murakami, Mario Tyago
Resumen
Nucleoside diphosphate kinases (NDKs; EC 2.7.4.6) play an essential role in the synthesis of nucleotides from intermediates in the salvage pathway in all parasitic trypanosomatids and their structural studies will be instrumental in shedding light on the biochemical machinery involved in the parasite life cycle and host-parasite interactions. In this work, NDKb from Leishmania major was overexpressed in Escherichia coli, purified to homogeneity and crystallized using the sitting-drop vapour-diffusion method. The NDK crystal diffracted to 2.2 angstrom resolution and belonged to the trigonal crystal system, with unit-cell parameters a = 114.2, c = 93.9 angstrom. Translation-function calculations yielded an unambiguous solution in the enantiomorphic space group P3(2)21. Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)