Thrombomodulin-independent activation of protein C and specificity of hemostatically active snake venom serine proteinases - Crystal structures of native and inhibited Agkistrodon contortrix contortrix protein C activator

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorMurakami, M. T.
dc.creatorArni, R. K.
dc.date2014-05-20T14:02:22Z
dc.date2016-10-25T17:09:04Z
dc.date2014-05-20T14:02:22Z
dc.date2016-10-25T17:09:04Z
dc.date2005-11-25
dc.date.accessioned2017-04-05T21:27:22Z
dc.date.available2017-04-05T21:27:22Z
dc.identifierJournal of Biological Chemistry. Bethesda: Amer Soc Biochemistry Molecular Biology Inc., v. 280, n. 47, p. 39309-39315, 2005.
dc.identifier0021-9258
dc.identifierhttp://hdl.handle.net/11449/21983
dc.identifierhttp://acervodigital.unesp.br/handle/11449/21983
dc.identifier10.1074/jbc.M508502200
dc.identifierWOS:000233362200050
dc.identifierhttp://dx.doi.org/10.1074/jbc.M508502200
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/867459
dc.descriptionProtein C activation initiated by the thrombin-thrombomodulin complex forms the major physiological anticoagulant pathway. Agkistrodon contortrix contortrix protein C activator, a glycosylated single-chain serine proteinase, activates protein C without relying on thrombomodulin. The crystal structures of native and inhibited Agkistrodon contortrix contortrix protein C activator determined at 1.65 and 1.54 angstrom resolutions, respectively, indicate the pivotal roles played by the positively charged belt and the strategic positioning of the three carbohydrate moieties surrounding the catalytic site in protein C recognition, binding, and activation. Structural changes in the benzamidine-inhibited enzyme suggest a probable function in allosteric regulation for the anion-binding site located in the C-terminal extension, which is fully conserved in snake venom serine proteinases, that preferentially binds Cl1- instead of SO42-.
dc.languageeng
dc.publisherAmer Soc Biochemistry Molecular Biology Inc
dc.relationJournal of Biological Chemistry
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.titleThrombomodulin-independent activation of protein C and specificity of hemostatically active snake venom serine proteinases - Crystal structures of native and inhibited Agkistrodon contortrix contortrix protein C activator
dc.typeOtro


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