dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorda Costa, Laiana Cristina
dc.creatorVilela de Azeredo-Oliveira, Maria Tercilia
dc.creatorTartarotti, Ester
dc.date2014-05-20T14:00:53Z
dc.date2014-05-20T14:00:53Z
dc.date2008-01-01
dc.date.accessioned2017-04-05T21:24:05Z
dc.date.available2017-04-05T21:24:05Z
dc.identifierGenetics and Molecular Biology. Ribeirao Pret: Soc Brasil Genetica, v. 31, n. 2, p. 438-444, 2008.
dc.identifier1415-4757
dc.identifierhttp://hdl.handle.net/11449/21510
dc.identifierS1415-47572008000300008
dc.identifierWOS:000256957500008
dc.identifierS1415-47572008000300008.pdf
dc.identifierhttp://dx.doi.org/10.1590/S1415-47572008000300008
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/867066
dc.descriptionTriatoma klugi is a Chagas disease vector in the Rio Grande do Sul State. Triatominae chromosomes are holocentric and sex chromosomes segregation is post-reductional. In this paper we describe the karyotype of male T. klugi and a meiotic analysis including the nucleolar behavior during spermatogenesis. Testis cells were analyzed after lacto-acetic orcein and silver nitrate staining. Two autosomes and the heterochromosomes presented nucleolar activity (Ag-NORs) during diplotene-diakinesis. The analysis of metaphase I and II revealed a karyotype with 2n = 20+XY. In metaphase I a prominent nucleolar mass was observed in the cell periphery and small silver grains were detected in metaphase II. During anaphase, the chromosomes segregated in parallel and a typical holocentric late migration behavior was observed. The restoration of the nucleolus was an important feature in this phase. During telophase nucleolar masses persisted and in early spermiogenesis the spermatids presented a small peripheral mass until elongation. The present study is a contribution to the study of chromatin behavior and nucleolar persistence in meiosis.
dc.languageeng
dc.publisherSoc Brasil Genetica
dc.relationGenetics and Molecular Biology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectTriatoma klugi
dc.subjectTriatomine
dc.subjectCytogenetic
dc.subjectNucleolus
dc.subjectKaryotype
dc.subjectMeiosis
dc.titleSpermatogenesis and nucleolar activity in Triatoma klugi (Triatomine, Heteroptera)
dc.typeOtro


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