Otro
Cytotoxicity of water-soluble fraction from biodiesel and its diesel blends to human cell lines
Registro en:
Ecotoxicology and Environmental Safety. San Diego: Academic Press Inc. Elsevier B.V., v. 74, n. 8, p. 2148-2155, 2011.
0147-6513
10.1016/j.ecoenv.2011.08.012
WOS:000296939700003
Autor
Leme, Daniela Morais
Grummt, Tamara
Heinze, Rita
Sehr, Andrea
Skerswetat, Matthias
Rodrigues de Marchi, Mary Rosa
Machado, Marcos Canto
de Oliveira, Danielle Palma
Mann-Morales, Maria Aparecida
Resumen
The designation of biodiesel as a green fuel has increased its commercialization and use, making its fate in the environment a matter of concern. Fuel spills constitute a major source of aquatic pollution and, like diesel spills, biodiesel can produce adverse effects on aquatic environments, animals and humans. The present study assessed cytotoxic effects of water systems contaminated with neat biodiesel and its diesel blends by means of different procedures on human T cell leukemia (Jurkat) and human hepatocellular carcinoma (HepG2) cells [detection of changes in mitochondrial membrane potential (Delta Psi(m)) using tetramethylrhodamine ethyl ester (TMRE), apoptosis recognition by Annexin V and impedance real-time cell analyzer (xCELLigence (TM) system)]. The data obtained showed concordance across the different bioassays, with cytotoxic effects observed as a dose-dependent response only for waters contaminated with pure diesel (D100) and B5 blend, which is characterized by a mixture of 95% diesel and 5% biodiesel. The data can also lead us to hypothesize that diesel accounts for the harmful effects observed, and that biodiesel does not worsen the impacts caused by diesel pollution. (C) 2011 Elsevier B.V. All rights reserved. Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)