In vitro study of CD133 human stem cells labeled with superparamagnetic iron oxide nanoparticles

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorGamarra, L. F.
dc.creatorPavon, L. F.
dc.creatorMarti, L. C.
dc.creatorPontuschka, W. M.
dc.creatorMamani, J. B.
dc.creatorCarneiro, S. M.
dc.creatorCamargo-Mathias, M. I.
dc.creatorMoreira-Filho, C. A.
dc.creatorAmaro, E.
dc.date2014-05-20T13:55:19Z
dc.date2016-10-25T17:05:06Z
dc.date2014-05-20T13:55:19Z
dc.date2016-10-25T17:05:06Z
dc.date2008-12-01
dc.date.accessioned2017-04-05T21:12:44Z
dc.date.available2017-04-05T21:12:44Z
dc.identifierNanomedicine-nanotechnology Biology and Medicine. Amsterdam: Elsevier B.V., v. 4, n. 4, p. 330-339, 2008.
dc.identifier1549-9634
dc.identifierhttp://hdl.handle.net/11449/19795
dc.identifierhttp://acervodigital.unesp.br/handle/11449/19795
dc.identifier10.1016/j.nano.2008.05.002
dc.identifierWOS:000262668600008
dc.identifierhttp://dx.doi.org/10.1016/j.nano.2008.05.002
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/865589
dc.descriptionSuperparamagnetic iron oxide nanoparticles (SPIONs) are applied in stem cell labeling because of their high magnetic susceptibility as compared with ordinary paramagnetic species, their low toxicity, and their ease of magnetic manipulation. The present work is the study of CD133(+) stem cell labeling by SPIONs coupled to a specific antibody (AC133), resulting in the antigenic labeling of the CD133+ stem cell, and a method was developed for the quantification of the SPION content per cell, necessary for molecular imaging optimization. Flow cytometry analysis established the efficiency of the selection process and helped determine that the CD133 cells selected by chromatographic affinity express the transmembrane glycoprotein CD133. The presence of antibodies coupled to the SPION, expressed in the cell membrane, was observed by transmission electron microscopy. Quantification of the SPION concentration in the marked cells using the ferromagnetic resonance technique resulted in a value of 1.70 x 10 (13) mol iron (9.5 pg) or 7.0 x 10 (6) nanoparticles per cell ( the measurement was carried out in a volume of 2 mu L containing about 6.16 x 10 5 pg iron, equivalent to 4.5 x 10 (11) SPIONs). (c) 2008 Elsevier B.V. All rights reserved.
dc.languageeng
dc.publisherElsevier B.V.
dc.relationNanomedicine-nanotechnology Biology and Medicine
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectCell labeling
dc.subjectMolecular imaging
dc.subjectFerromagnetic resonance
dc.subjectCD133
dc.subjectSPIONs
dc.titleIn vitro study of CD133 human stem cells labeled with superparamagnetic iron oxide nanoparticles
dc.typeOtro


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