Multiple bradykinin-related peptlides from the capture web of the spider Nephila clavipes (Araneae, Tetragnatidae)

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorVolsi, ECFR
dc.creatorMendes, M. A.
dc.creatorMarques, M. R.
dc.creatordos Santos, L. D.
dc.creatorSantos, K. S.
dc.creatorde Souza, B. M.
dc.creatorBabieri, E. F.
dc.creatorPalma, Mario Sergio
dc.date2014-05-20T13:54:26Z
dc.date2016-10-25T17:04:35Z
dc.date2014-05-20T13:54:26Z
dc.date2016-10-25T17:04:35Z
dc.date2006-04-01
dc.date.accessioned2017-04-05T21:10:54Z
dc.date.available2017-04-05T21:10:54Z
dc.identifierPeptides. New York: Elsevier B.V., v. 27, n. 4, p. 690-697, 2006.
dc.identifier0196-9781
dc.identifierhttp://hdl.handle.net/11449/19463
dc.identifierhttp://acervodigital.unesp.br/handle/11449/19463
dc.identifier10.1016/j.peptides.2005.08.011
dc.identifierWOS:000237047000012
dc.identifierhttp://dx.doi.org/10.1016/j.peptides.2005.08.011
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/865341
dc.descriptionThree bradykinin-related peptides (nephilakinins-I to -III) and bradykinin itself were isolated from the aqueous washing extract of the capture web of the spider Nephila clavipes by gel permeation chromatography on a Sephacryl S-100 column, followed by chromatography in a Hi-Trap Sephadex-G25 Superfine column. The novel peptides occur-red in low concentrations and were sequenced through ESI-MS/MS analysis: nephilakinin-I (G-P-N-P-G-F-S-P-F-R-NH2), nephilakinin-Il (E-A-P-P-G-F-S-P-F-R-NH2) and nephilakinin-III (P-S-P-P-G-F-S-P-F-R-NH2)- Synthetic peptides replicated the novel bradykinin-related peptides, which were submitted to biological characterizations. Nephilakinins were shown to cause constriction on isolated rat ileum preparations and relaxation on rat duodenum muscle preparations at amounts higher than bradykinin; apparently these peptides constitute B-2-type agonists of ileal and duodenal smooth muscles. All peptides including the bradykinin were moderately lethal to honeybees. These bradykinin peptides may be related to the predation of insects by the webs of N. clauipes. (c) 2005 Elsevier B.V. All rights reserved.
dc.languageeng
dc.publisherElsevier B.V.
dc.relationPeptides
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectspider toxins
dc.subjectbradykinin-related peptides
dc.subjectmuscle contraction/relaxation
dc.subjectmass spectrometrypeptide sequencing
dc.subjectpeptide synthesis
dc.titleMultiple bradykinin-related peptlides from the capture web of the spider Nephila clavipes (Araneae, Tetragnatidae)
dc.typeOtro


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