Quantification of bovine cytokine gene expression using real-time RT-PCR methodology

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorZaros, Lilian Giotto
dc.creatorBricarello, Patrízia Ana
dc.creatorAmarante, Alessandro Francisco Talamini do
dc.creatorCoutinho, Luiz Lehmann
dc.date2014-05-20T13:52:56Z
dc.date2014-05-20T13:52:56Z
dc.date2007-01-01
dc.date.accessioned2017-04-05T21:07:48Z
dc.date.available2017-04-05T21:07:48Z
dc.identifierGenetics and Molecular Biology. Sociedade Brasileira de Genética, v. 30, n. 3, p. 575-579, 2007.
dc.identifier1415-4757
dc.identifierhttp://hdl.handle.net/11449/18890
dc.identifier10.1590/S1415-47572007000400012
dc.identifierS1415-47572007000400012
dc.identifierS1415-47572007000400012.pdf
dc.identifierhttp://dx.doi.org/10.1590/S1415-47572007000400012
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/864920
dc.descriptionT cells produce cytokines that affect host response to infection. This paper reports real-time RT-PCR conditions and validation steps for accurate quantification of Bos indicus cytokines, interleukin (IL)-2, IL-4, IL-8, IL12p-35, IL-13, tumoral necrosis factor (TNF)-alpha, interferon (IFN)-gamma, monocyte chemoattractant proteins (MCP)-1 and MCP-2, and the glycoprotein mucin (MUC)-1 in two groups of Nelore cattle, one resistant and the other susceptible to gastrointestinal nematode infections. RPL-19 was shown to be an ideal internal control gene, since its expression was constant across treatments and presented lower variation when compared to the GAPDH gene. The optimized conditions established in the present study can be used to determine the immune response of cattle under different experimental conditions, such as viral, bacterial and parasite infections.
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.languageeng
dc.publisherSociedade Brasileira de Genética
dc.relationGenetics and Molecular Biology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectcytokine gene expression
dc.subjectreal-time RT-PCR
dc.subjectcattle
dc.subjectgastrointestinal nematodes
dc.subjectbovine cytokines
dc.titleQuantification of bovine cytokine gene expression using real-time RT-PCR methodology
dc.typeOtro


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