Sugar digestion in mosquitoes: Identification and characterization of three midgut alpha-glucosidases of the neo-tropical malaria vector Anopheles aquasalis (Diptera : Culicidae)

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorSouza-Neto, Jayme A.
dc.creatorMachado, Fabio P.
dc.creatorLima, Jose B.
dc.creatorValle, Denise
dc.creatorRibolla, Paulo E. M.
dc.date2014-05-20T13:52:44Z
dc.date2016-10-25T17:03:37Z
dc.date2014-05-20T13:52:44Z
dc.date2016-10-25T17:03:37Z
dc.date2007-08-01
dc.date.accessioned2017-04-05T21:07:26Z
dc.date.available2017-04-05T21:07:26Z
dc.identifierComparative Biochemistry and Physiology A-molecular & Integrative Physiology. New York: Elsevier B.V., v. 147, n. 4, p. 993-1000, 2007.
dc.identifier1095-6433
dc.identifierhttp://hdl.handle.net/11449/18840
dc.identifierhttp://acervodigital.unesp.br/handle/11449/18840
dc.identifier10.1016/j.cbpa.2007.03.008
dc.identifierWOS:000247717000022
dc.identifierhttp://dx.doi.org/10.1016/j.cbpa.2007.03.008
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/864872
dc.descriptionDietary carbohydrates provide an important source of energy for flight, and contribute to longevity and fecundity of mosquitoes. The most common sugar mosquitoes ingest is sucrose, and digestion of this substance is carried out mainly by alpha-glucosidases. In the current work, we tested the efficiency of sucrose on Anopheles aquasalis female diet. The best longevity (days) was reached when sugar was available in the diet, whereas most only blood fed females were dead 6 days after emergence. Three alpha-glucosidase isoforms were detected in the adult female midgut, named alpha Glu1, alpha Glu2 and alpha Glu3. These are acidic alpha-glucosidases with optima pH around pH 5.5. alpha Glu1 and alpha Glu2 are present in both secreted and membrane-bound forms, whereas alpha Glu3 only in anchored to membranes. The alpha-glucosidase activity is concentrated mainly in the posterior midgut (70%), both in non-fed or 10% sucrose fed females. The single form of these a-glucosidases seemed to be similar to 70 kDa polypeptides, although alpha Glu2 is presented in >= 600 kDa self-aggregates. K, values of alpha Glu1, alpha Glu2 and alpha Glu3 differed significantly from each other, supporting the statement that three alpha-glucosidases are produced in the female midgut. Together, all data suggest that sugar is an essential component of A. aquasalis female diet. In addition, alpha-glucosidases are synthesized in the same place where sucrose is digested and absorbed, the midgut. (c) 2007 Elsevier B.V. All rights reserved.
dc.languageeng
dc.publisherElsevier B.V.
dc.relationComparative Biochemistry and Physiology A-molecular & Integrative Physiology
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectAnopheles aquasalis
dc.subjectmalaria
dc.subjectmosquito
dc.subjectsucrose
dc.subjectsurvivorship
dc.subjectmidgut
dc.subjectdigestion
dc.subjectalpha-glucosidase
dc.titleSugar digestion in mosquitoes: Identification and characterization of three midgut alpha-glucosidases of the neo-tropical malaria vector Anopheles aquasalis (Diptera : Culicidae)
dc.typeOtro


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