Isolation and characterization of a satellite DNA family in Achirus lineatus (Teleostei : Pleuronectiformes : Achiridae)

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorde Azevedo, MFC
dc.creatorOliveira, C.
dc.creatorMartins, C.
dc.creatorWasko, A. P.
dc.creatorForesti, F.
dc.date2014-05-20T13:51:26Z
dc.date2016-10-25T17:03:00Z
dc.date2014-05-20T13:51:26Z
dc.date2016-10-25T17:03:00Z
dc.date2005-11-01
dc.date.accessioned2017-04-05T21:05:13Z
dc.date.available2017-04-05T21:05:13Z
dc.identifierGenetica. Dordrecht: Springer, v. 125, n. 2-3, p. 205-210, 2005.
dc.identifier0016-6707
dc.identifierhttp://hdl.handle.net/11449/18387
dc.identifierhttp://acervodigital.unesp.br/handle/11449/18387
dc.identifier10.1007/s10709-005-8419-0
dc.identifierWOS:000232841800008
dc.identifierhttp://dx.doi.org/10.1007/s10709-005-8419-0
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/864573
dc.descriptionAgarose gels stained with Ethidium bromide and Southern blot experiments of HindIII-digested genomic DNA of Achirus lineatus evidenced the presence of monomers and multimers of a DNA segment of about 200 bp, named here Al-HindIII sequence. No signals were observed in Southern blot experiments with genomic DNA of other flatfish species. The DNA sequencing of four recombinant clones showed that Al-HindIII sequences had 204 bp and were 63.72% AT-rich. FISH experiments using a Al-HindIII sequence as probe showed bright signals in the centromeric position of all chromosomes of A. lineatus.
dc.languageeng
dc.publisherSpringer
dc.relationGenetica
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectchromosome structure
dc.subjectfluorescent in situ hybridization (FISH)
dc.subjectPleuronectiformes
dc.subjectsatellite DNA
dc.titleIsolation and characterization of a satellite DNA family in Achirus lineatus (Teleostei : Pleuronectiformes : Achiridae)
dc.typeOtro


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