Molecular cytogenetic analysis of heterochromatin in the chromosomes of tilapia, Oreochromis niloticus (Teleostei : Cichlidae)

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorOliveira, C.
dc.creatorWright, J. M.
dc.date2014-05-20T13:51:16Z
dc.date2016-10-25T17:02:55Z
dc.date2014-05-20T13:51:16Z
dc.date2016-10-25T17:02:55Z
dc.date1998-04-01
dc.date.accessioned2017-04-05T21:04:54Z
dc.date.available2017-04-05T21:04:54Z
dc.identifierChromosome Research. Dordrecht: Kluwer Academic Publ, v. 6, n. 3, p. 205-211, 1998.
dc.identifier0967-3849
dc.identifierhttp://hdl.handle.net/11449/18303
dc.identifierhttp://acervodigital.unesp.br/handle/11449/18303
dc.identifier10.1023/A:1009211701829
dc.identifierWOS:000074376600008
dc.identifierhttp://dx.doi.org/10.1023/A:1009211701829
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/864529
dc.descriptionThe structure of the heterochromatic bands in mitotic chromosomes of the important tropical aquaculture species of tilapia, Oreochromis niloticus, was investigated by the combination of the C-banding technique, chromosomal digestion with two restriction endonucleases and fluorescence in situ hybridization (FISH) of two satellite DNAs (SATA and SATB). The tilapia chromosomes presented heterochromatic bands in the centromeres and in the short arms of almost all chromosomes that were differentially digested by the restriction endonucleases HaeIII and EcoRI. FISH of SATA showed that the satellite sequence is distributed in the centromeric region of all chromosomes of tilapia. FISH also revealed an intense hybridization signal for SATB in only one chromosome pair, but less intense signals were also present in several other pairs. The digestion of tilapia chromosomes by HaeIII and EcoRI was positively correlated with the position of SATA and SATB in chromosomes as revealed by FISH. The results obtained may be useful in future molecular and genetic studies of tilapias.
dc.languageeng
dc.publisherKluwer Academic Publ
dc.relationChromosome Research
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectchromosome structure
dc.subjectfish cytogenetics
dc.subjectfluorescence in situ hybridization
dc.subjectheterochromatin
dc.subjectsatellite DNA
dc.titleMolecular cytogenetic analysis of heterochromatin in the chromosomes of tilapia, Oreochromis niloticus (Teleostei : Cichlidae)
dc.typeOtro


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