PRP8 intein in Ajellomycetaceae family pathogens: Sequence analysis, splicing evaluation and homing endonuclease activity

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorTheodoro, Raquel Cordeiro
dc.creatorVolkmann, Gerrit
dc.creatorLiu, Xiang-Qin
dc.creatorBagagli, Eduardo
dc.date2014-05-20T13:51:01Z
dc.date2016-10-25T17:02:47Z
dc.date2014-05-20T13:51:01Z
dc.date2016-10-25T17:02:47Z
dc.date2011-02-01
dc.date.accessioned2017-04-05T21:04:22Z
dc.date.available2017-04-05T21:04:22Z
dc.identifierFungal Genetics and Biology. San Diego: Academic Press Inc. Elsevier B.V., v. 48, n. 2, p. 80-91, 2011.
dc.identifier1087-1845
dc.identifierhttp://hdl.handle.net/11449/18216
dc.identifierhttp://acervodigital.unesp.br/handle/11449/18216
dc.identifier10.1016/j.fgb.2010.07.010
dc.identifierWOS:000286487600002
dc.identifierhttp://dx.doi.org/10.1016/j.fgb.2010.07.010
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/864464
dc.descriptionInteins are intervening sequences that are transcribed and translated with flanking host protein sequences and then self-excised by protein splicing. Bi-functional inteins also contain a homing endonuclease responsible for their genetic mobility. The PRP8 intein, the most widespread among fungi, occurs in important pathogens such as Histoplasma capsulatum and Paracoccidioides brasiliensis, from the Ajellomycetaceae family. Herein, we describe the bi-functional PRP8 intein in two other Ajellomycetacean pathogens, Blastomyces dermatitidis and Emmonsia parva. Sequence analysis and experimental evidence suggest that the homing endonuclease from PbrPRP8 is inactive. The splicing activity of the PRP8 intein from the B. dermatitidis, E. parva and P. brasiliensis species complex was demonstrated in a non-native protein context in Escherichia coil. Since the PRP8 intein is located in a functionally essential nuclear protein, it can be considered a promising therapeutic target for anti-fungal drugs, because inhibition of intein splicing should inhibit proliferation of intein-containing pathogens. (C) 2010 Published by Elsevier B.V.
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.languageeng
dc.publisherAcademic Press Inc. Elsevier B.V.
dc.relationFungal Genetics and Biology
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectAjellomycetaceae
dc.subjectIntein
dc.subjectPRP8
dc.subjectSplicing
dc.subjectHoming endonuclease
dc.titlePRP8 intein in Ajellomycetaceae family pathogens: Sequence analysis, splicing evaluation and homing endonuclease activity
dc.typeOtro


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