Otro
Pharmacological dose of alpha-tocopherol induces cardiotoxicity in Wistar rats determined by echocardiography and histology
Registro en:
Human & Experimental Toxicology. London: Sage Publications Ltd, v. 30, n. 10, p. 1540-1548, 2011.
0960-3271
10.1177/0960327110392401
WOS:000295812200013
Autor
Nascimento, Maria Carolina M. O.
Matsubara, Beatriz Bojikian
Matsubara, Luiz Shiguero
Corrêa, Camila Renata
Pereira, Elenize J.
Moreira, Priscila L.
Carvalho, Flavio A.
Burini, Caio H.
Padovani, Carlos Roberto
Yeum, Kyung-Jin
Ferreira, Ana Lúcia dos Anjos
Resumen
The effect of pharmacological dose of cc-tocopherol on heart health was determined in Wistar rats. Animals were randomly assigned to either C (control, n = II) or E (cc-tocopherol, n = II) group. Animals received corn oil (C) or alpha-tocopherol dissolved in corn oil (250 mg alpha-tocopherol/[kg body wt/day]) (E) by gavage for a 7-week period. Rats underwent echocardiogram and were analyzed for cardiomyocyte histology and cardiac cc-tocopherol absorption at the end of the study period. As compared to the C group, alpha-tocopherol-supplemented group showed significantly (p < 0.05) lower body weight (E, 412.8 g vs C, 480.3 g) and total cardiac weight (E, 0.94 g vs C, 1.08 g); cardiomyocyte histological impairment; smaller left ventricle (LV) (LV end-diastolic diameter (E, 7.22 mm vs C, 7.37 mm), lower LV systolic [left ventricle fractional shortening (E, 47.6% vs C, 53.6%) and ejection fraction ratio (E, 85.4 vs C, 89.9)] and diastolic [early peak velocities of diastolic transmitral flow (E, 64.6 cm/sec vs C, 75.1 cm/sec)] function. The alpha-tocopherol uptake in target tissue was confirmed by determination of alpha-tocopherol concentration medians in cardiac tissue (E, 109.91 nmol/kg vs C, 52.09 nmol/kg). The current study indicates that pharmacological dose of alpha-tocopherol supplementation can induce cardiotoxicity in healthy rats. Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)