Otro
Adherence in vitro of Candida albicans to plasma treated acrylic resin. Effect of plasma parameters, surface roughness and salivary pellicle
Registro en:
Archives of Oral Biology. Oxford: Pergamon-Elsevier B.V. Ltd, v. 55, n. 10, p. 763-770, 2010.
0003-9969
10.1016/j.archoralbio.2010.06.015
WOS:000282399100006
Autor
Zamperini, Camila Andrade
Machado, Ana Lucia
Vergani, Carlos Eduardo
Pavarina, Ana Claudia
Giampaolo, Eunice Terezinha
da Cruz, Nilson Cristino
Resumen
The adhesion of Candida albicans to surfaces is the prerequisite for occurrence of denture stomatitis.Objective: Hence, this study investigated if surface modifications with plasma treatments could reduce the adherence of C. albicans to a denture base resin.Methods: Specimens (n = 180)with roughened and smooth surfaces were made and divided into five groups: control specimens were left untreated; experimental groups specimens were submitted to plasma treatments to obtain surfaces with different hydrophobicity (Ar/50 W; ArO(2)/70 W; AAt/130 W) or incorporation of fluorine (Ar/SF(6)70 W). Contact angle measurements were performed immediately after the treatments and after immersion in water for 48 h. For each group, half of the specimens were incubated with saliva prior to the adhesion assay. The number of adherent yeasts was evaluated by XTT reduction method.Results: For the experimental groups, there was significant change in the mean contact angle after 48 h of immersion in water. Groups ArO(2)/70 W and ArSF(6)/70 W showed significantly lower absorbance readings than the other groups, regardless the presence or absence of saliva and surface roughness.Conclusions: Results demonstrated that ArO(2)/70 W and ArSF(6)/70 W plasma treatments showed promising potential for reducing the adherence of C. albicans to denture base resins. (C) 2010 Elsevier Ltd. All rights reserved. Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)