Several species of Phaeoacremonium have been associated with young grapevine decline in major production regions of California, South Africa, Australia, Europe and now in Brazil. Infection of Phaeoacremonium spp. causes darkening of xylem vessels with production of tyloses and dark gummy masses resulting in occlusion of xylem vessels. The fungus isolated from these samples produced colonies that rarely exceed 15 to 20 mm diameter after several weeks growth at 20°C. In this study the effects of culture media and temperature on mycelial growth of Phaeaocremonium angustius CNPUV 533 were evaluated.Resumo P.S. I-2.