Interleukin-4 and interleukin-13 inhibit the expression of leukemia inhibitory factor and interleukin-11 in fibroblasts

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorSouza, Pedro P. C.
dc.creatorPalmqvist, Py
dc.creatorLundberg, Pernilla
dc.creatorLundgren, Inger
dc.creatorHanstrom, Lennart
dc.creatorSouza, Joao A. C.
dc.creatorConaway, H. Herschel
dc.creatorLerner, Ulf H.
dc.date2014-05-20T13:45:59Z
dc.date2016-10-25T16:59:51Z
dc.date2014-05-20T13:45:59Z
dc.date2016-10-25T16:59:51Z
dc.date2012-01-01
dc.date.accessioned2017-04-05T20:53:55Z
dc.date.available2017-04-05T20:53:55Z
dc.identifierMolecular Immunology. Oxford: Pergamon-Elsevier B.V. Ltd, v. 49, n. 4, p. 601-610, 2012.
dc.identifier0161-5890
dc.identifierhttp://hdl.handle.net/11449/16233
dc.identifierhttp://acervodigital.unesp.br/handle/11449/16233
dc.identifier10.1016/j.molimm.2011.10.009
dc.identifierWOS:000300212100005
dc.identifierhttp://dx.doi.org/10.1016/j.molimm.2011.10.009
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/863075
dc.descriptionCytokines produced by inflammatory or resident mesenchymal cells play important modulatory roles in the pathogenesis of inflammation induced bone loss. In the present study, the effects of IL-4 and IL-13 on the expression of three osteotropic cytokines in the IL-6 family expressed in human gingival fibroblasts were studied. IL-4R alpha and IL-13R alpha 1 mRNA were constitutively expressed in human gingival fibroblasts. The inflammatory cytokines IL-1 beta and TNF-alpha increased expression of IL-5, LIF, and IL-11 mRNA and protein in the gingival fibroblasts. Addition of IL-4 or IL-13 had no effect on IL-6 expression, but significantly inhibited LIF and IL-11 mRNA and protein stimulated by IL-1 beta and TNF-alpha. No involvement of NF-kappa B or STAT1 was observed in the inhibition. STAT6 was phosphorylated at Y641 by treatment with IL-4 and knockdown of STAT6 with siRNA decreased the inhibition of IL-11 and LIF expression by IL-4 in IL-1 beta and TNF-alpha stimulated cells. This study suggests that activation of STAT6 by IL-4 and IL-13, through type 2 IL-4 receptors, inhibits production of IL-11 and LIF stimulated by IL-1 beta and TNF-alpha in human gingival fibroblasts. A negative modulatory role of IL-4 and IL-13 in osteotropic cytokine production could be a mechanism playing an important inhibitory role in inflammation induced periodontitis. (C) 2011 Elsevier Ltd. All rights reserved.
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.languageeng
dc.publisherPergamon-Elsevier B.V. Ltd
dc.relationMolecular Immunology
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectInterleukin-4
dc.subjectInterleukin-6
dc.subjectGingival fibroblasts
dc.subjectBone resorption
dc.titleInterleukin-4 and interleukin-13 inhibit the expression of leukemia inhibitory factor and interleukin-11 in fibroblasts
dc.typeOtro


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