Detection of the single nucleotide polymorphism (rs2227307) in the human interleukin 8 gene using a pcr-rflp assay

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorKim, Yeon Jung
dc.creatorViana, Aline Cavalcanti
dc.creatorTfaile Corbi, Samia Cruz
dc.creatorde Carvalho Curtis, Karen Maria
dc.creatorRenzi, Rivelto
dc.creatorOrrico, Silvana Regina Perez
dc.creatorCirelli, Joni Augusto
dc.creatorScarel-Caminaga, Raquel Mantuaneli
dc.date2013-09-30T18:32:04Z
dc.date2014-05-20T13:45:32Z
dc.date2013-09-30T18:32:04Z
dc.date2014-05-20T13:45:32Z
dc.date2009-11-01
dc.date.accessioned2017-04-05T20:52:48Z
dc.date.available2017-04-05T20:52:48Z
dc.identifierBioscience Journal. Uberlandia: Universidade Federal de Uberlândia (UFU), v. 25, n. 6, p. 136-142, 2009.
dc.identifier1516-3725
dc.identifierhttp://hdl.handle.net/11449/16026
dc.identifierWOS:000272788800015
dc.identifierWOS000272788800015.pdf
dc.identifierhttp://www.seer.ufu.br/index.php/biosciencejournal/article/view/7041
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/862927
dc.descriptionInterleukin 8 (IL-8) is a chemokine that acts as a potent chemoattractant for neutrophils. Single nucleotide polymorphisms ( SNPs) in the human IL8 gene have been investigated in many disease association studies. We have developed a different PCR-RFLP (Polymerase Chain Reaction - Restriction Fragment of Length Polymorphism) assay for genotyping the SNP (rs2227307) in the IL8 gene. This method was used for typing 147 white healthy Brazilian individuals, whose DNA was obtained from buccal epithelial cells and extracted with phenol: chloroform: isoamyl alcohol. Genomic DNA was amplified by PCR using a conventional thermal cycler. The PCR products ( 573 bp) were submitted to RFLP reactions. The RFLP fragments were analyzed in a 4% agarose gel stained with ethidium bromide. The genotype distribution observed in this study was consistent with the assumption of Hardy-Weinberg equilibrium and was similar (p=0.30) to those reported for other white populations in the SNP Database of the National Center for Biotechnology Information (NCBI). Because the PCR-RFLP method presented here was efficient, low cost, reproducible and convenient for laboratories with a limited level of technology worldwide, it should be useful for genotyping in case-control association or population genetic studies.
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.languageeng
dc.publisherUniversidade Federal de Uberlândia (UFU)
dc.relationBioscience Journal
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectSingle Nucleotide Polymorphism
dc.subjectInterleukin 8
dc.subjectPCR-RFLP assay
dc.titleDetection of the single nucleotide polymorphism (rs2227307) in the human interleukin 8 gene using a pcr-rflp assay
dc.typeOtro


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