Otro
Correlation between koilocytes and human papillomavirus detection by PCR in oral and oropharynx squamous cell carcinoma biopsies
Registro en:
Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 106, n. 2, p. 166-169, 2011.
0074-0276
10.1590/S0074-02762011000200008
S0074-02762011000200008
WOS:000289961100008
S0074-02762011000200008.pdf
Autor
Miyahara, Glauco Issamu
Simonato, Luciana Estevam
Mattar, Neivio José
Camilo Jr, Deolino João
Biasoli, Eder Ricardo
Resumen
The purpose of this study was to compare the histopathological analysis with polymerase chain reaction (PCR) methods to predict the presence of human papillomavirus (HPV) infection in oral squamous cell carcinoma biopsies. Eighty-three paraffin-embedded tissue specimens from patients with oropharynx and mouth floor squamous cell carcinoma were submitted to histopathological analysis under light microscopy, specifically for the determination of the presence of koilocytes. Subsequently, DNA was purified from the same paraffin-embedded specimens and submitted to PCR. Fisher's exact test showed no statistically significant correlation between the two methods. The results suggest that the presence of koilocytes is unreliable for the detection of HPV presence in oral and oropharynx squamous cell carcinoma. Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)