| dc.contributor | Universidade Estadual Paulista (UNESP) | |
| dc.creator | Cardoso, T. | |
| dc.creator | Pilz, D. | |
| dc.date | 2014-02-26T17:20:32Z | |
| dc.date | 2014-05-20T13:42:21Z | |
| dc.date | 2016-10-25T16:57:11Z | |
| dc.date | 2014-02-26T17:20:32Z | |
| dc.date | 2014-05-20T13:42:21Z | |
| dc.date | 2016-10-25T16:57:11Z | |
| dc.date | 2004-11-15 | |
| dc.date.accessioned | 2017-04-05T20:44:09Z | |
| dc.date.available | 2017-04-05T20:44:09Z | |
| dc.identifier | Veterinary Microbiology. Amsterdam: Elsevier B.V., v. 103, n. 3-4, p. 161-167, 2004. | |
| dc.identifier | 0378-1135 | |
| dc.identifier | http://hdl.handle.net/11449/14729 | |
| dc.identifier | http://acervodigital.unesp.br/handle/11449/14729 | |
| dc.identifier | 10.1016/j.vetmic.2004.07.017 | |
| dc.identifier | WOS:000225111500004 | |
| dc.identifier | http://dx.doi.org/10.1016/j.vetmic.2004.07.017 | |
| dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/861831 | |
| dc.description | A simple, sensitive and specific plaque assay protocol for the detection of wild type rabies virus in different species is described using confluent monolayers of chicken embryo cells in 6-well plates. Plaques are produced after application of either agarose or Sephadex G-100 overlay onto cell monolayers and incubation for 96 h after virus infection at 37 degreesC. The parameters affecting plaque appearance include cell seeding concentration, overlay composition and time of incubation after infection. Optimal conditions are seeding at a concentration of 4 x 10(6) cell/cm(3), incubation at 37 degreesC in 5% CO2 atmosphere during 96 h, using either 1% agarose or 2% Sephadex G-100 overlays. The described plaque assay would be a new valuable too] in conducting various quantitative investigations, since the chicken embryo cells are susceptible to rabies virus infection from all species studied. (C) 2004 Elsevier B.V. All rights reserved. | |
| dc.language | eng | |
| dc.publisher | Elsevier B.V. | |
| dc.relation | Veterinary Microbiology | |
| dc.rights | info:eu-repo/semantics/closedAccess | |
| dc.subject | rabies virus | |
| dc.subject | plaque assay | |
| dc.subject | chicken embryo related cells | |
| dc.title | Wild rabies virus detection by plaque assay from naturally infected brains in different species | |
| dc.type | Otro | |
