Ovulation rate and its relationship with follicle diameter and gene expression of the LH receptor (LHR) in Nelore cows

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorSimoes, Renato A. L.
dc.creatorSatrapa, Rafael A.
dc.creatorRosa, Fernando S.
dc.creatorPiagentini, Marcelo
dc.creatorCastilho, Anthony C. S.
dc.creatorEreno, Ronaldo Luiz
dc.creatorTrinca, Luzia A.
dc.creatorNogueira, Marcelo Fábio Gouveia
dc.creatorBuratini, Jose
dc.creatorBarros, Ciro M.
dc.date2013-09-30T18:28:35Z
dc.date2014-05-20T13:42:03Z
dc.date2016-10-25T16:56:57Z
dc.date2013-09-30T18:28:35Z
dc.date2014-05-20T13:42:03Z
dc.date2016-10-25T16:56:57Z
dc.date2012-01-01
dc.date.accessioned2017-04-05T20:43:18Z
dc.date.available2017-04-05T20:43:18Z
dc.identifierTheriogenology. New York: Elsevier B.V., v. 77, n. 1, p. 139-147, 2012.
dc.identifier0093-691X
dc.identifierhttp://hdl.handle.net/11449/14604
dc.identifierhttp://acervodigital.unesp.br/handle/11449/14604
dc.identifier10.1016/j.theriogenology.2011.07.027
dc.identifierWOS:000298336300017
dc.identifierhttp://dx.doi.org/10.1016/j.theriogenology.2011.07.027
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/861718
dc.descriptionThe objective was to determine the relationship among the diameter of ovarian follicles, ovulation rate, and gene expression of the LH receptor (LHR) in Nelore cattle. In Experiment 1, ovulation was synchronized in 53 Nelore cows. Three days after ovulation, ovaries were assessed with ultrasonography, all cows were given 6.25 mg LH im, and they were allocated into three groups, according to diameter of their largest ovarian follicle: G1 (7.0-8.0 mm); G2 (8.1-9.0 mm); and G3 (9.1-10.0 mm). For these three groups, ovulation rates were 9, 36, and 90%, respectively, (P < 0.03; each rate differed significantly from the other two). In Experiment 2, granulosa and theca cells were subjected to total RNA extraction, and gene expression of the LHR was determined by RT-PCR. Follicles were allocated in three groups based on their diameter (similar to the Experiment 1), which were denoted Groups A, B, and C. Expression of the LHR gene in granulosa cells was lower in Group A than Group C (P < 0.05). However, there were no significant differences among groups in expression of the LHR gene in theca cells. We concluded that ovulatory capacity in Nelore cattle was related to increased follicular diameter and expression of the LHR gene in granulosa cells. (C) 2012 Elsevier B.V. All rights reserved.
dc.languageeng
dc.publisherElsevier B.V.
dc.relationTheriogenology
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectFollicle
dc.subjectGene expression
dc.subjectLH receptor
dc.subjectOvulation
dc.subjectCattle
dc.titleOvulation rate and its relationship with follicle diameter and gene expression of the LH receptor (LHR) in Nelore cows
dc.typeOtro


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