dc.contributor | Universidade do Estado do Rio de Janeiro (UERJ) | |
dc.contributor | FIOCRUZ | |
dc.contributor | Universidade Federal de São Paulo (UNIFESP) | |
dc.creator | Guimaraes, M. L. | |
dc.creator | Bastos, F. I. | |
dc.creator | Telles, P. R. | |
dc.creator | Galvao-Castro, B. | |
dc.creator | Diaz, R. S. | |
dc.creator | Bongertz, V | |
dc.creator | Morgado, M. G. | |
dc.date.accessioned | 2016-01-24T12:31:23Z | |
dc.date.accessioned | 2023-09-04T18:20:09Z | |
dc.date.available | 2016-01-24T12:31:23Z | |
dc.date.available | 2023-09-04T18:20:09Z | |
dc.date.created | 2016-01-24T12:31:23Z | |
dc.date.issued | 2001-05-01 | |
dc.identifier | Journal of Clinical Virology. Amsterdam: Elsevier B.V., v. 21, n. 2, p. 143-151, 2001. | |
dc.identifier | 1386-6532 | |
dc.identifier | http://repositorio.unifesp.br/handle/11600/26544 | |
dc.identifier | 10.1016/S1386-6532(01)00158-5 | |
dc.identifier | WOS:000168906900005 | |
dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/8613316 | |
dc.description.abstract | Background: Retrovirus infections among injecting drug users (IDUs), a core at-risk population for both HIV-1 and HTLV-I/II infections in Brazil, were assessed within an ongoing cooperative research. Objective: the study assessed the seroprevalences of HIV-1 and HTLV-I/II infections, as well as the prevalence of HIV-1 subtypes in a sample of IDUs from Rio de Janeiro, Brazil. An attempt to evaluate HIV incidence was carried out using a dual 'sensitive/less sensitive' testing strategy. Study design: Cross-sectional evaluation of 175 IDUs. Serostatus for HIV-1 and HTLV-I,II were established by enzyme-linked immunosorbent assays, and confirmed by western blot. the dual testing strategy aimed to estimate HIV-1 incidence rates. Differentiation between HTLV-I and -II was performed by western blot. DNA samples were polymerase chain reaction amplified by a nested protocol, and HIV-1 subtyping was determined by heteroduplex mobility assay. Results: Forty-six and 29 samples were found to be, respectively, positive for HIV-1 and HTLV-I/II, 15 of them co-infected by both viruses. Among HTLV-I/II-infected patients, 75.9% were infected by HTLV-I. Thirty-one HIV samples were identified as B subtype, with seven of them showing the typical 'Brazilian B' pattern in the gp120 V3 loop, and ten were identified as F subtype. the use of less sensitive assays for HIV infection wrongly identified a deeply immunocompromised patient as an incident case. Conclusion: Moderately high seroprevalences were found for both HIV-1 and HTLV-I/II infections, HIV-1/HTLV-I co-infections being of special concern. A non-statistically significant higher prevalence of F subtype was observed, when compared with the distribution of F/B subtypes among Brazilian patients from other exposure categories. No recent HIV-1 infections were detected, but a limitation of the 'sensitive/less-sensitive' testing strategy was made evident. (C) 2001 Elsevier Science B.V. All rights reserved. | |
dc.language | eng | |
dc.publisher | Elsevier B.V. | |
dc.relation | Journal of Clinical Virology | |
dc.rights | http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy | |
dc.rights | Acesso restrito | |
dc.subject | injecting drug users | |
dc.subject | HIV | |
dc.subject | HTLV | |
dc.subject | HIV/HTLV co-infection | |
dc.subject | sensitive/less sensitive dual testing strategy for HIV infection | |
dc.title | Retrovirus infections in a sample of injecting drug users in Rio de Janeiro City, Brazil: prevalence of HIV-1 subtypes, and co-infection with HTLV-I/II | |
dc.type | Artigo | |