Artigo
Biochemical Characterization of an In-House Coccidioides Antigen: Perspectives for the Immunodiagnosis of Coccidioidomycosis
Fecha
2012-07-01Registro en:
Molecules. Basel: Mdpi Ag, v. 17, n. 7, p. 7854-7863, 2012.
1420-3049
WOS000306752700018.pdf
10.3390/molecules17077854
WOS:000306752700018
Autor
Moreira Filho, Renato Evando
Bandeira, Silviane Praciano
Nogueira Brillhante, Raimunda Samia
Gadelha Rocha, Marcos Fabio
Vasconcelos, Ilka Maria
Pereira, Mirella Leite
Collares Maia Castelo-Branco, Debora de Souza
Castro Rocha, Francisco Airton
Camargo, Zoilo Pires de [UNIFESP]
Ramos, Marcio Viana
Cordeiro, Rossana de Aguiar
Costa Sidrim, Jose Julio
Institución
Resumen
The objective of this study was to evaluate the reactivity of an in-house antigen, extracted from a strain of C. posadasii isolated in northeastern Brazil, by radial immunodiffusion and Western blotting, as well as to establish its biochemical characterization. the protein antigen was initially extracted with the use of solid ammonium sulfate and characterized by 1-D electrophoresis. Subsequently, it was tested by means of double radial immunodiffusion and Western blotting. A positive reaction was observed against the antigen by both immunodiagnostic techniques tested on sera from patients suffering from coccidioidomycosis. Besides this, two immunoreactive protein bands were observed and were revealed to be a beta-glucosidase and a glutamine synthetase after sequencing of the respective N-terminal regions. Our in-house Coccidioides antigen can be promising as a quick and low-cost diagnostic tool without the risk of direct manipulation of the microorganism.