Artigo
Effect of estrogen therapy on vascular perlecan and metalloproteinases 2 and 9 in castrated rats
Fecha
2013-02-01Registro en:
Climacteric. London: Informa Healthcare, v. 16, n. 1, p. 147-153, 2013.
1369-7137
10.3109/13697137.2012.667173
WOS:000313681300021
Autor
Pompei, L. M.
Steiner, M. L.
Theodoro, T. R.
Souza, P. Z.
Romanini, A. C. A.
Coulson-Thomas, Vivien Jane [UNIFESP]
Pinhal, M. A. S.
Fernandes, C. E.
Institución
Resumen
Aim To study the effects of estrogen therapy on the expression of matrix metalloproteinases 2 and 9 (MMP-2 and MMP-9) and perlecan in the vascular wall.Methods Twenty 180-day-old Wistar rats were castrated and treated 1 week later for a period of 4 weeks with one of the following: (1) placebo; (2) 0.5 mu g/day estradiol benzoate (E2B); (3) 5 mu g/day E2B; (4) 50 mu g/day E2B. A fifth group consisted of rats that had not been castrated. Following treatment, expression of MMP-2 and MMP-9 mRNA (MMP-2([RNA]) and MMP-9([RNA]), respectively) was analyzed by real-time PCR, and expression of MMP-2 (MMP-2([IH])), MMP-9 (MMP-9([IH])) and perlecan was quantified by immunohistochemistry, in carotid walls.Results There were no differences among castrated groups for MMP-2([RNA]) (p = 0.1969) and for MMP-9([RNA]) (p = 0.1828); however, a correlation was observed between E 2 B dose and MMP-9([RNA]) levels (r = 0.471, p = 0.018). Differences among groups were observed for MMP-2([IH]), MMP-9([IH]) and perlecan (p < 0.0001), wherein higher levels were observed in animals treated with estrogen therapy, correlating with E2B doses in the case of MMP-9 (r = 0.441, p = 0.026) and perlecan (r = 0.574, p = 0.005).Conclusions Estrogen therapy correlates with higher levels of MMP-2, MMP-9 and perlecan in the extracellular matrix of carotid walls in castrated rats, in a dose-dependent manner. There was a dose-response effect of E2B on the expression of MMP-9 mRNA and, possibly, MMP-2 mRNA.