Differentiation of Klebsiella pneumoniae carbapenemase (KPC) variants by pyrosequencing

Monteiro, Jussimara [UNIFESP]; Widen, Raymond H.; Pignatari, Antonio C. C. [UNIFESP]; Kubasek, Carly; Silbert, Suzane

Artigo

Fecha

2014-05-01

Registro en:

Journal of Microbiological Methods. Amsterdam: Elsevier B.V., v. 100, p. 42-45, 2014.

0167-7012

http://repositorio.unifesp.br/handle/11600/37736

10.1016/j.mimet.2014.02.013

WOS:000335631200007

https://repositorioslatinoamericanos.uchile.cl/handle/2250/8612946

Autor

Monteiro, Jussimara [UNIFESP]

Widen, Raymond H.

Pignatari, Antonio C. C. [UNIFESP]

Kubasek, Carly

Silbert, Suzane

Institución

Universidade Federal de São Paulo (Brasil)

Resumen

A fast and reliable protocol using the pyrosequencing technique was developed to identify 11 different types of the KPC enzyme. A total of 65 b/alpha(Kpc) positive bacterial isolates were tested and characterized. in the end, the pyrosequencing proved to be a powerful tool for epidemiological studies of KPC producer isolates. (c) 2014 Elsevier B.V. All rights reserved.

Materias

Pyrosequencing

Real-time PCR

Klebsiella pneumoniae carbapenemase

KPC

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