Otro
Metalloproteinases 2 and-9 activity during promotion and progression stages of rat liver carcinogenesis
Registro en:
Journal of Molecular Histology. Dordrecht: Springer, v. 40, n. 1, p. 1-11, 2009.
1567-2379
10.1007/s10735-008-9206-x
WOS:000264485300001
Autor
Furtado, Kelly Silva
Pires, Paulo Wagner
Justulin, Luis Antonio
Rodrigues, Maria Aparecida Marchesan
Felisbino, Sergio Luis
Barbisan, Luis Fernando
Resumen
Activity of metalloproteinases 2 and 9 (MMP-2 and 9) during promotion and progression of rat liver carcinogenesis was investigated in a modified resistant hepatocyte model. Development of preneoplastic liver lesions positive for glutathione S-transferase 7-7-(GST-P 7-7-positive PNL) and tumors besides hepatocytes positive for proliferating cell nuclear antigen (PCNA) were quantified and compared to MMP-2 and-9 activity using gelatin zymography. Marked increases in GST-P 7-7-positive PNL development, PCNA labeling indices, MMP-2 (pro, intermediate and active forms) and pro-MMP-9 activity were observed after proliferative stimulus induced by 2-acetylaminofluorene (2-AAF) exposure cycles. After 2-AAF withdrawal, increase in MMP-2 activity was detected only in neoplastic mixed lesions, whereas active MMP-9 was increased in both PLN and neoplastic tissues. Our findings suggest that MMP-2 may be associated with proliferative events induced by 2-AAF rather than with selective growth of PNL and that MMP-9 could be associated with progression of PNL and neoplastic mixed lesions. Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)