article
Anti-TNF-α agent tamarind kunitz trypsin inhibitor improves lipid profile of Wistar rats presenting dyslipidemia and diet-induced obesity regardless of PPAR-γ induction
Registro en:
10.3390/nu11030512
Autor
Morais, Ana Heloneida de Araújo
Carvalho, Fabiana Maria Coimbra de
Lima, Vanessa Cristina Oliveira de
Costa, Izael Souza
Luz, Anna Beatriz Santana
Ladd, Fernando Vagner Lobo
Serquiz, Alexandre Coelho
Bortolin, Raul Hernandes
Silbiger, Vivian Nogueira
Maciel, Bruna Leal Lima
Santos, Elizeu Antunes dos
Resumen
The increasing prevalence of obesity and, consequently, chronic inflammation and its complications has increased the search for new treatment methods. The effect of the purified tamarind seed trypsin inhibitor (TTIp) on metabolic alterations in Wistar rats with obesity and dyslipidemia was evaluated. Three groups of animals with obesity and dyslipidemia were formed, consuming a high glycemic index and glycemic load (HGLI) diet, for 10 days: Obese/HGLI diet; Obese/standard diet; Obese/HGLI diet + TTIp (730 µg/kg); and one eutrophic group of animals was fed a standard diet. Rats were evaluated daily for food intake and weight gain. On the 11th day, animals were anesthetized and sacrificed for blood and visceral adipose tissue collection. TTIp treated animals presented significantly lower food intake than the untreated group (p = 0.0065), TG (76.20 ± 18.73 mg/dL) and VLDL-C (15.24 ± 3.75 mg/dL). Plasma concentrations and TNF-α
mRNA expression in visceral adipose tissue also decreased in obese animals treated with TTIp (p < 0.05 and p = 0.025, respectively) with a negative immunostaining. We conclude that TTIp presented anti-TNF-α activity and an improved lipid profile of Wistar rats with dyslipidemia and obesity induced by a high glycemic index and load diet regardless of PPAR-γ induction.