| dc.contributor | Universidade Estadual Paulista (UNESP) | |
| dc.creator | Menezes, Manoel Lima de | |
| dc.creator | Demarchi, ACCO | |
| dc.date | 2014-05-20T13:26:49Z | |
| dc.date | 2016-10-25T16:46:53Z | |
| dc.date | 2014-05-20T13:26:49Z | |
| dc.date | 2016-10-25T16:46:53Z | |
| dc.date | 1998-01-01 | |
| dc.date.accessioned | 2017-04-05T20:06:39Z | |
| dc.date.available | 2017-04-05T20:06:39Z | |
| dc.identifier | Journal of Liquid Chromatography & Related Technologies. New York: Marcel Dekker Inc., v. 21, n. 15, p. 2355-2363, 1998. | |
| dc.identifier | 1082-6076 | |
| dc.identifier | http://hdl.handle.net/11449/8703 | |
| dc.identifier | http://acervodigital.unesp.br/handle/11449/8703 | |
| dc.identifier | 10.1080/10826079808000543 | |
| dc.identifier | WOS:000075691900008 | |
| dc.identifier | http://dx.doi.org/10.1080/10826079808000543 | |
| dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/857012 | |
| dc.description | This method has been developed for extraction and determination of phenol in a urine sample by high performance liquid chromatography.After acid hydrolysis, the free phenol was extracted with isoamyl alcohol solvent, followed by back extraction with 0.5 mol.L-1 sodium hydroxide solution and analyzed by an isocratic HPLC Varian System, equipped with reverse-phase column (MicroPak-C-18). The mobile phase was acetonitrile in 0.01 mol.L-1 hydrochloric acid solution, (20:80 v/v), and at a now-rate of 1.0 mL.min-1. The chromatogram was monitored at 220 nm in room temperature. The identification was based on retention time and the quantification was performed by automatic peak-area determination, corrected for the external standards method.The recovery was higher than 99.5 % for phenol and reproducibility of method was shown to be 2.3% standard deviation and 5.6% coefficient of variance (n=20). The limit detection was 0.05 mgL(-1) and a range of 0.05 to 20.0 mgL(-1) of phenol for linearity. | |
| dc.language | eng | |
| dc.publisher | Marcel Dekker Inc | |
| dc.relation | Journal of Liquid Chromatography & Related Technologies | |
| dc.rights | info:eu-repo/semantics/closedAccess | |
| dc.title | Off line extraction of phenol from human urine sample with isoamyl alcohol and determination by HPLC | |
| dc.type | Otro | |
