Tetracaine stimulates insulin secretion through the mobilization of Ca2+ from thapsigargin- and IP3-insensitive Ca2+ reservoir in pancreatic beta-cells

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorBosqueiro, JR
dc.creatorCarneiro, E. M.
dc.creatorBordin, S.
dc.creatorBoschero, A. C.
dc.date2014-05-20T13:25:37Z
dc.date2016-10-25T16:46:02Z
dc.date2014-05-20T13:25:37Z
dc.date2016-10-25T16:46:02Z
dc.date2000-06-01
dc.date.accessioned2017-04-05T20:03:31Z
dc.date.available2017-04-05T20:03:31Z
dc.identifierCanadian Journal of Physiology and Pharmacology. Ottawa: Natl Research Council Canada, v. 78, n. 6, p. 462-468, 2000.
dc.identifier0008-4212
dc.identifierhttp://hdl.handle.net/11449/8140
dc.identifierhttp://acervodigital.unesp.br/handle/11449/8140
dc.identifier10.1139/cjpp-78-6-462
dc.identifierWOS:000087770700004
dc.identifierhttp://dx.doi.org/10.1139/cjpp-78-6-462
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/856605
dc.descriptionThe effect of tetracaine on Ca-45 efflux, cytoplasmic Ca2+ concentration [Ca2+](i), and insulin secretion in isolated pancreatic islets and beta-cells was studied. In the absence of external Ca2+, tetracaine (0.1-2.0 mM) increased the Ca-45 efflux from isolated islets in a dose-dependant manner. Tetracaine did not affect the increase in Ca-45 efflux caused by 50 mM K+ or by the association of carbachol (0.2 mM) and 50 mM K+. Tetracaine permanently increased the [Ca2+](i) in isolated beta-cells in Ca2+-free medium enriched with 2.8 mM glucose and 25 mu M D-600 (methoxiverapamil). This effect was also observed in the presence of 10 mM caffeine or 1 mu M thapsigargin. In the presence of 16.7 mM glucose, tetracaine transiently increased the insulin secretion from islets perfused in the absence and presence of external Ca2+. These data indicate that tetracaine mobilises Ca2+ from a thapsigargin-insensitive store and stimulates insulin secretion in the absence of extracellular Ca2+. The increase in Ca-45 efflux caused by high concentrations of K+ and by carbachol indicates that tetracaine did not interfere with a cation or inositol triphosphate sensitive Ca2+ pool in beta-cells.
dc.languageeng
dc.publisherNatl Research Council Canada
dc.relationCanadian Journal of Physiology and Pharmacology
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjecttetracaine
dc.subjectbeta-cells
dc.subjectinsulin secretion
dc.subjectCa2+
dc.titleTetracaine stimulates insulin secretion through the mobilization of Ca2+ from thapsigargin- and IP3-insensitive Ca2+ reservoir in pancreatic beta-cells
dc.typeOtro


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