The control of production processes can assist in the standardization of variability, reducing waste, and improving the quality of a service or product. Thus, this study aimed to analyze the non-conformities in a production system of forest seedlings from Atlantic Forest aiming at the standardization of the production system and adjustments for field cultivation. The definition of the attributes was made through a technical visit to the forestry nursery to know the location and the production process of the seedlings. For the process evaluation, statistical process control tools were used. The non-conformities analyzed were: coiled root growth, disintegrated substrates of plants, presence of roots fixed to the ground, presence of phytopathogen attack symptoms and/or herbivory and symptoms of nutritional deficiency. In general, variability was detected in the production process, compromising the success in planting the seedlings in the field, as well as their quality.