Otro
First Identification of Culex flavivirus (Flaviviridae) in Brazil
Registro en:
Intervirology. Basel: Karger, v. 55, n. 6, p. 475-483, 2012.
0300-5526
10.1159/000337166
WOS:000309519100009
Autor
Machado, Daiane Cristina
Mondini, Adriano
Santana, Vinicius dos Santos
Kamiya Yonamine, Patricia Tiemi
Chiaravalloti Neto, Francisco
de Andrade Zanotto, Paolo Marinho
Nogueira, Mauricio Lacerda
Resumen
Introduction: Culex flavivirus (CxFV) was first isolated in 2007 from Culex pipiens in Japan and then identified in several other countries. Characterization of the CxFV showed that all strains are related to the cell fusing agent virus. In this manuscript we report the first identification of CxFV in South America. Material and Methods: We have collected Culex sp. mosquitoes using BG-Sentinel traps and manual aspirators. They were pooled according to genus, species, sex and location. Viral RNA was extracted and multiplex nested PCR was performed to test the presence of Flavivirus. The positive samples were isolated in C6/36 cells and sequenced for phylogenetic analyses. Results: 265 female Culex mosquitoes pooled in 83 pools were tested with specific CxFV, Saint Louis encephalitis virus (SLEV) and West Nile virus (WNV) primers. Our sequence data indicated maximum sequence similarity of 97% with CxFV. Discussion: In this study we report the circulation of CxFV in an urban setting where SLEV had previously caused an outbreak. In terms of public health, this is an important finding due to the assumption that the previous exposition of mosquitoes to CxFV might lessen the susceptibility of these mosquitoes to other flaviviruses. Copyright (C) 2012 S. Karger AG, Basel Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)