Free radical scavenging profile and myeloperoxidase inhibition of extracts from antidiabetic plants: Bauhinia forficata and Cissus sicyoides

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorKhalil, Najeh M.
dc.creatorPepato, Maria Teresa
dc.creatorBrunetti, Iguatemy Lourenço
dc.date2014-05-20T13:24:00Z
dc.date2014-05-20T13:24:00Z
dc.date2008-01-01
dc.date.accessioned2017-04-05T19:59:03Z
dc.date.available2017-04-05T19:59:03Z
dc.identifierBiological Research. Santiago: Soc Biolgia Chile, v. 41, n. 2, p. 165-171, 2008.
dc.identifier0716-9760
dc.identifierhttp://hdl.handle.net/11449/7354
dc.identifierS0716-97602008000200006
dc.identifierWOS:000259578800006
dc.identifierS0716-97602008000200006-en.pdf
dc.identifierhttp://dx.doi.org/10.4067/S0716-97602008000200006
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/856044
dc.descriptionThere is abundant evidence that reactive oxygen species are implicated in several physiological and pathological processes. To protect biological targets from oxidative damage. antioxidants must react with radicals and other reactive species faster than biological substrates do. The aim of the present study was to determine the in vitro antioxidant activity of aqueous extracts from leaves of Bauhinia forficata Link (Fabaceae - Caesalpinioideae) and Cissus sicyoides L. (Vitaceae) (two medicinal plants used popularly in the control of diabetes mellitus), using several different assay systems, namely, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) decolorization. superoxide anion radical (O-2 center dot-) scavenging and myeloperoxidase (MPO) activity. In the ABTS assay for total antioxidant activity, B. forficata showed IC50 8.00 +/- 0.07 mu g/mL, while C. sicyoides showed IC50 13.0 +/- 0.2 mu g/mL. However, the extract of C. sicyoides had a stronger effect on O-2 center dot- (IC50 60.0 +/- 2.3 mu p/mL) than the extract of B. forficata (IC50 90.0 +/- 4.4 mu g/mL). B. forficata also had a stronger inhibitory effect on MPO activity, as measured by guaiacol oxidation, than C. sicyoides. These results indicate that aqueous extracts of leaves of B. forficata and C. sicyoides are a potential source of natural antioxidants and may be helpful in the prevention of diabetic complications associated with oxidative stress.
dc.languageeng
dc.publisherSoc Biolgia Chile
dc.relationBiological Research
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectABTS decolorization assay
dc.subjectantioxidant activity
dc.subjectsuperoxide anion scavenging activity
dc.titleFree radical scavenging profile and myeloperoxidase inhibition of extracts from antidiabetic plants: Bauhinia forficata and Cissus sicyoides
dc.typeOtro


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