Inhibitory Effect of Gallic Acid and Its Esters on 2,2 '-Azobis(2-amidinopropane)hydrochloride (AAPH)-Induced Hemolysis and Depletion of Intracellular Glutathione in Erythrocytes

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorXimenes, Valdecir F.
dc.creatorLopes, Mariana G.
dc.creatorPetronio, Maicon Segalla
dc.creatorRegasini, Luis Octavio
dc.creatorSiqueira Silva, Dulce H.
dc.creatorda Fonseca, Luis M.
dc.date2014-05-20T13:23:53Z
dc.date2016-10-25T16:44:46Z
dc.date2014-05-20T13:23:53Z
dc.date2016-10-25T16:44:46Z
dc.date2010-05-12
dc.date.accessioned2017-04-05T19:58:46Z
dc.date.available2017-04-05T19:58:46Z
dc.identifierJournal of Agricultural and Food Chemistry. Washington: Amer Chemical Soc, v. 58, n. 9, p. 5355-5362, 2010.
dc.identifier0021-8561
dc.identifierhttp://hdl.handle.net/11449/7286
dc.identifierhttp://acervodigital.unesp.br/handle/11449/7286
dc.identifier10.1021/jf100233y
dc.identifierWOS:000277237500021
dc.identifierhttp://dx.doi.org/10.1021/jf100233y
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/856005
dc.descriptionThe protective effect of gallic acid and its esters, methyl, propyl, and lauryl gallate, against 2,2'-azobis(2-amidinopropane)hydrochloride (AAPH)-induced hemolysis and depletion of intracellular glutathione (GSH) in erythrocytes was studied. The inhibition of hemolysis was dose-dependent, and the esters were significantly more effective than gallic acid. Gallic acid and its esters were compared with regard to their reactivity to free radicals, using the DPPH and AAPH/pyranine free-cell assays, and no significant difference was obtained. Gallic acid and its esters not only failed to inhibit the depletion of intracellular GSH in erythrocytes induced by AAPH but exacerbated it. Similarly, the oxidation of GSH by AAPH or horseradish peroxidase/H(2)O(2) in cell-free systems was exacerbated by gallic acid or gallates. This property could be involved in the recent findings on proapoptotic and pro-oxidant activities of gallates in tumor cells. We provide evidence that lipophilicity and not only radical scavenger potency is an important factor regarding the efficiency of antihemolytic substances.
dc.languageeng
dc.publisherAmer Chemical Soc
dc.relationJournal of Agricultural and Food Chemistry
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectGallic acid
dc.subjectgallates
dc.subjecterythrocytes
dc.subjecthemolysis
dc.subjectglutathione
dc.subjectperoxyl radical
dc.subjectantioxidant
dc.subjectpro-oxidant activity
dc.titleInhibitory Effect of Gallic Acid and Its Esters on 2,2 '-Azobis(2-amidinopropane)hydrochloride (AAPH)-Induced Hemolysis and Depletion of Intracellular Glutathione in Erythrocytes
dc.typeOtro


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