| dc.creator | Garc?a, Camilo | |
| dc.creator | Navarro, Fredy | |
| dc.creator | Ru?z-Le?n, Domingo | |
| dc.creator | Garc?a-Beltr?n, Olimpo | |
| dc.creator | Aguirre, Mar?a Jes?s | |
| dc.date | 2019-03-11T16:24:16Z | |
| dc.date | 2019-03-11T16:24:16Z | |
| dc.date | 2017-01-10 | |
| dc.date.accessioned | 2023-08-31T19:12:48Z | |
| dc.date.available | 2023-08-31T19:12:48Z | |
| dc.identifier | Garc?a, C., Navarro, F., Ru?z-Le?n, D. et al. J Solid State Electrochem (2017) 21: 243. | |
| dc.identifier | 1432-8488 | |
| dc.identifier | https://link.springer.com/article/10.1007/s10008-016-3367-4 | |
| dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/8556360 | |
| dc.description | In this work, a cationic porphyrin, ascribed as [Ttolyl(P-(C6H5)3)4]4+, where Ttolyl = 5,10,15,20 tetrakistolylporphyrin, was electrostatically assembled with CdTe quantum dots capped with glutathione (GSH) of diameter ~3.28 nm via a layer-by-layer methodology. This multilayer assembly was evaluated as a biocompatible interface with synergic effects for calf thymus double-stranded DNA (CT DNA) immobilization, considering that the hybrid assembly contains a cationic porphyrin with a high binding constant for CT DNA, and quantum dots with polypeptide as a capping agent. The multilayer assembly, ascribed as ITO/{[Ttolyl(P-(C6H5)3)4]4+/CdTe} n (n = 1?5), was characterized by UV-Vis spectroscopy, cyclic voltammetry, and electrochemical impedance spectroscopy (EIS). The last technique was explored in order to check the adsorption of CT DNA onto the multilayer porphyrin/quantum dot assembly. The difference in electron transfer resistance (?R ct) obtained after CT DNA incubations showed the best result for a specific multilayer assembly, n = 3. {[Ttolyl(P-(C6H5)3)4]4+/CdTe}3 showed a good correlation of ?R ct with the logarithmic concentration of CT DNA, in the range 1.0 ? 10?10 to 1.0 ? 10?6 M with a limit of detection (LOD) of 1.5 ? 10?12 M. Conversely, when the {[Ttolyl(P-(C6H5)3)4]4+/CdTe}3 system was incubated with calf thymus single-stranded DNA and salmon testes DNA, no significant difference in ?R ct was observed. We conclude that the newly described {[Ttolyl(P-(C6H5)3)4]4+/CdTe} n system is a choice method for the impedimetric determination of CT DNA. | |
| dc.language | en | |
| dc.publisher | Journal of Solid State Electrochemistry | |
| dc.subject | Cationic porphyrins | |
| dc.subject | Quantum dots | |
| dc.subject | DNA-porphyrin interactions | |
| dc.subject | Layer by layer assemblies | |
| dc.subject | Calf thymus DNA determination | |
| dc.title | Impedimetric evaluation of hybrid cationic porphyrin/quantum dot multilayer assemblies: a biocompatible interface for calf thymus DNA immobilization | |
| dc.type | Article | |
