Screening Botryosphaeria species for lipases: Production of lipase by Botryosphaeria ribis EC-01 grown on soybean oil and other carbon sources

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorMessias, Josana M.
dc.creatorda Costa, Bruna Z.
dc.creatorde Lima, Valeria M. G.
dc.creatorDekker, Robert F. H.
dc.creatorRezende, Maria I.
dc.creatorKrieger, Nadia
dc.creatorBarbosa, Aneli M.
dc.date2014-05-20T13:22:12Z
dc.date2016-10-25T16:43:28Z
dc.date2014-05-20T13:22:12Z
dc.date2016-10-25T16:43:28Z
dc.date2009-12-01
dc.date.accessioned2017-04-05T19:53:49Z
dc.date.available2017-04-05T19:53:49Z
dc.identifierEnzyme and Microbial Technology. New York: Elsevier B.V., v. 45, n. 6-7, p. 426-431, 2009.
dc.identifier0141-0229
dc.identifierhttp://hdl.handle.net/11449/6492
dc.identifierhttp://acervodigital.unesp.br/handle/11449/6492
dc.identifier10.1016/j.enzmictec.2009.08.013
dc.identifierWOS:000271260700003
dc.identifierhttp://dx.doi.org/10.1016/j.enzmictec.2009.08.013
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/855378
dc.descriptionNine isolates of Botryosphaeria spp. were screened for lipases when cultivated on eight different plant seed oils and glycerol, and all produced lipases Botryosphaeria ribis EC-01 produced highest lipase litres on soybean oil and glycerol, while eight isolates of Botryosphaeria rhodina produced significantly lower enzyme tares. B ribis EC-01 produced lipase when grown on different fatty acids. surfactants, carbohydrates and triacylglycerols, with highest enzyme titres produced on Triton X-100-emulsified stearic (3167 U/mL), palmitic (283.5 U/mL) and oleic (247.4 U/mg) acids, and soybean oil (105 6U/mL), as well as castor oil (191.2 U/mg); an enhancement of 9-fold over soybean oil-grown cultures. Glycerol was also a good substrate for lipase production. The crude lipase extract was optimally active at pH 8.0 and 55 C, stable between 30 and 55 C and pH 1-10, and tolerant to 50%(v/v) glycerol, methanol and ethanol. The crude lipase showed affinity for substrates of short, average and long-chain fatty acids (different esters of p-nitrophenol and triacylglycerols). Zymograms developed with 4-methylumbelliferyl-butyrate showed two bands of lipolytic activity at 45 and 15 kDa. This is the first report on the production of lipases by B. ribis grown on these different carbon sources. (C) 2009 Elsevier B.V. All rights reserved.
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.languageeng
dc.publisherElsevier B.V.
dc.relationEnzyme and Microbial Technology
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectLipase
dc.subjectBotryosphaeria ribis EC-01
dc.subjectBotryosphaeria rhodina
dc.subjectCastor oil
dc.subjectSoybean oil and glycerol
dc.subjectThermal and pH stability
dc.subjectStability in organic solvents
dc.titleScreening Botryosphaeria species for lipases: Production of lipase by Botryosphaeria ribis EC-01 grown on soybean oil and other carbon sources
dc.typeOtro


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