Valorization of an Andean crop (Yacon) through the production of a yeast cell-bound phytase.

dc.contributor
dc.creatorConde Molina, Débora
dc.creatorNovelli Poisson, Guido
dc.creatorKronberg, Florencia
dc.creatorGalvagno, Miguel Angel
dc.date2022-02-18T20:08:58Z
dc.date2022-02-18T20:08:58Z
dc.date2021-08
dc.date.accessioned2023-08-31T14:30:53Z
dc.date.available2023-08-31T14:30:53Z
dc.identifierhttp://hdl.handle.net/20.500.12272/5984
dc.identifier10.1016
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8547864
dc.descriptionThe suitability of the yeast Schwanniomyces (f. Debaryomyces) occidentalis to produce phytase activity on a Smallanthus sonchifolius (yacon) extract-based medium (YSM) was studied. Optimal conditions for phytase production were evaluated in YSM at 28 ◦C and pH 5.5, with an inoculum of 107 cells/mL, in shaken flasks, using a Box-Benkhen optimization design, and analyzed by response surface methodology. Phytase location was determined by subcellular fractioning and protoplast formation. Maximum phytase titres were obtained with 0.11% w/v yacon reducing sugar, 0.65% w/v (NH4)2SO4 and 16 h of fermentation. The maximum phytase activity attained under these conditions was over 6-fold higher than in non-optimized medium. Scaling-up in a STR-type bioreactor further increased phytase productivity by 1.5-fold. Phytase produced was associated to the cell wall fraction, and presented optimal temperature and pH range values of 75–80 ◦C and 4.0–5.0, respectively, retaining 80% of its activity at 80 ◦C for 40 min. Progressively increasing phosphorus concentration in the medium inhibited enzyme production, up to 60% with 0.1% w/v KH2PO4. Yeast biomass, phytase production and production kinetics in YSM were similar to those produced in a cane molasses-based medium. Yacon resulted in a promising alternative feed stock to produce a yeast-bound phytase potentially applicable in the feed industry as a nutritional additive.
dc.descriptionFil: Conde Molina, Débora. Universidad Tecnológica Nacional. Facultad Regional Delta. Departamento de Ingeniería Química. Laboratorio de Biotecnología; Argentina.
dc.descriptionFil: Novelli Poisson, Guido. Universidad de Buenos Aires. Facultad de Ingeniería. Departamento de Ingeniería Química. Laboratorio de Microbiología Industrial; Argentina.
dc.descriptionFil: Kronberg, Florencia. Universidad de Buenos Aires. Facultad de Agronomía. INBA-CONICET; Argentina.
dc.descriptionFil: Galvagno, Miguel Angel. Instituto de Investigaciones Biotecnológicas (IIBIO); Argentina.
dc.descriptionFil: Galvagno, Miguel Angel. Universidad Nacional de San Martín (UNSAM).; Argentina.
dc.descriptionFil: Galvagno, Miguel Angel. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina.
dc.descriptionPeer Reviewed
dc.formatpdf
dc.languageeng
dc.relationhttps://www.sciencedirect.com/science/article/abs/pii/S1878818121002127?via%3Dihub
dc.rightsopenAccess
dc.rightshttp://creativecommons.org/licenses/by-nc-sa/4.0/
dc.rightsAtribución-NoComercial-CompartirIgual 4.0 Internacional
dc.rightsLos autores
dc.rightsAtribución – No Comercial – Sin Obra Derivada (by-nc-nd)
dc.subjectUTN
dc.subjectFacultad Regional Delta
dc.subjectFeed industry
dc.subjectNutritional additive
dc.subjectYeast Phytase Schwanniomyces occidentalis
dc.subjectSmallanthus sonchifolius (Yacon)
dc.titleValorization of an Andean crop (Yacon) through the production of a yeast cell-bound phytase.
dc.typeinfo:eu-repo/semantics/article
dc.typeacceptedVersion


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