info:eu-repo/semantics/article
Enhancement of soybean nodulation by seed treatment with non–thermal plasmas
Registro en:
Scientific Reports | (2020) 10:4917
10.1038/s41598-020-61913-3
Autor
Pérez Pizá, María Cecilia
Cejas, Ezequiel
Zilli, Carla
Prevosto, Leandro
Mancinelli, Beatriz
Santa Cruz, Diego
Yannarelli, Gustavo
Balestrasse, Karina
Institución
Resumen
Soybean (Glycine max (L.) Merrill) is one of the most important crops worldwide providing dietary
protein and vegetable oil. Most of the nitrogen required by the crop is supplied through biological
N2 fxation. Non-thermal plasma is a fast, economical, and environmental-friendly technology that
can improve seed quality, plant growth, and crop yield. Soybean seeds were exposed to a dielectric
barrier discharge plasma operating at atmospheric pressure air with superimposed fows of O2 or N2 as
carrying gases. An arrangement of a thin phenolic sheet covered by polyester flms was employed as
an insulating barrier. We focused on the ability of plasma to improve soybean nodulation and biological
nitrogen fxation. The total number of nodules and their weight were signifcantly higher in plants
grown from treated seeds than in control. Plasma treatments incremented 1.6 fold the nitrogenase
activity in nodules, while leghaemoglobin content was increased two times, indicating that nodules
were fxing nitrogen more actively than control. Accordingly, the nitrogen content in nodules and the
aerial part of plants increased by 64% and 23%, respectively. Our results were supported by biometrical
parameters. The results suggested that diferent mechanisms are involved in soybean nodulation
improvement. Therefore, the root contents of isofavonoids, glutathione, auxin and cytokinin, and
expansin (GmEXP1) gene expression were determined. We consider this emerging technology is a
suitable pre-sowing seed treatment. Universidad Tecnológica Nacional (PID 5447), Agencia Nacional
de Promoción Científica y Tecnológica (PICT 2015 N°1553), Universidad de Buenos Aires (UBACYT
20020160100031). K. B., C. Z. and L. P. are members of CONICET. E. C. thanks CONICET for his doctoral fellowship. M.C.P.P thanks Agencia Nacional de Promoción Científca y Tecnológica for her doctoral fellowship. We thank María Marta Cafaro (CONICET – Facultad de Agronomía, Universidad de Buenos Aires, Argentina) for her valuable assistance and for the constructive suggestions made during the assays planning. We thank also to Patricia del Fueyo (Seed Laboratory - Universidad de Buenos Aires, Facultad de Agronomía, Argentine) who provided scientifc support that greatly assisted this research. We would like to ofer special thanks to Dr. Beatriz Mancinelli who, although no longer with us, continues inspiring the group by her example and dedication. Fil: Pérez Pizá, María Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina. Fil: Cejas, Ezequiel. Universidad Tecnológica Nacional Facultad Regional Venado Tuerto. Departamento Ingeniería Electromecánica. Grupo de Descargas Eléctricas; Argentina. Fil: Zilli, Carla. Consejo Nacional de Investigaciones Científicas y Técnicas. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina. Fil: Prevosto, Leandro. Universidad Tecnológica Nacional Facultad Regional Venado Tuerto. Departamento Ingeniería Electromecánica. Grupo de Descargas Eléctricas; Argentina. Fil: Mancinelli, Beatriz. Universidad Tecnológica Nacional Facultad Regional Venado Tuerto. Departamento Ingeniería Electromecánica. Grupo de Descargas Eléctricas; Argentina. Fil: Santa Cruz, Diego. CONICET. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad Favaloro. Laboratorio de Regulación Génica y Células Madre. Instituto de Medicina Traslacional, Trasplante y Bioingeniería; Argentina. Fil: Yannarelli, Gustavo. CONICET. Universidad Favaloro. Laboratorio de Regulación Génica y Células Madre. Instituto de Medicina Traslacional, Trasplante y Bioingeniería; Argentina. Fil: Balestrasse, Karina. CONICET. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina. Peer Reviewed