Providing clean drinking water is a great challenge worldwide, especially for low-income countries where the access to safe water is limited. During the last decade, new biotechnological approaches have been explored to improve water management. Among them, the use of antimicrobial nanoparticles for designing innovative centralized and decentralized (point-of-use) water treatment systems for microbial decontamination has received considerable attention. Herein, antimicrobial lignin capped silver nanoparticles (AgLNP) were embedded on residual cork pieces using highintensity ultrasound coupled with laccase-mediated grafting to obtain biofunctionalized nanomaterial. The developed AgLNP-coated cork proved to be highly efficient to drastically reduce the number of viable Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus in liquid medium. Additionally, the coated-cork was characterized using FTIR-ATR spectroscopy and SEM imaging, and further used as a filter bed in a point-of-use device for water disinfection. The constructed water filtering system significantly reduced the amount of viable E. coli and resistant Bacillus cereus spores from filtered water operating at increasing residence times of 1, 4, 6, 16, 24, and 48 h. Therefore, the presented results prove that the obtained cork-based antimicrobial nanocomposite material could be used as a filtering medium for the development of water filtration system to control pathogen dissemination.Fil: Bermeo, Lizeth. Unesco Chair on Sustainability. Universitat Politècnica de Catalunya. Escola Superior d'Enginyeries Industrial, Aeroespacial i Audiovisual de Terrassa (ESEIAAT); Spain.Fil: Pérez, Leonardo Martín. Unesco Chair on Sustainability. Universitat Politècnica de Catalunya. Escola Superior d'Enginyeries Industrial, Aeroespacial i Audiovisual de Terrassa (ESEIAAT); Spain.Fil: Forés, Eva. Unesco Chair on Sustainability. Universitat Politècnica de Catalunya. Escola Superior d'Enginyeries Industrial, Aeroespacial i Audiovisual de Terrassa (ESEIAAT); Spain.Fil: Morató, Jordi. Unesco Chair on Sustainability. Universitat Politècnica de Catalunya. Escola Superior d'Enginyeries Industrial, Aeroespacial i Audiovisual de Terrassa (ESEIAAT); Spain.Fil: Bermeo, Lizeth. Universidad Del Cauca. Facultad de Ingeniería Civil. Departamento de Ingeniería Ambiental. Grupo de Investigación Ciencia e Ingeniería en Sistemas Ambientales (GCISA); Colombia.Fil: Casas Zapata, Juan C. Universidad Del Cauca. Facultad de Ingeniería Civil. Departamento de Ingeniería Ambiental. Grupo de Investigación Ciencia e Ingeniería en Sistemas Ambientales (GCISA); Colombia.Fil: Ivanova, Kristina. Universitat Politècnica de Catalunya. Departament d’Enginyeria Química. Grup de Biotecnologia Molecular i Industrial; Spain.Fil: Pérez Rafael, Silvia. Universitat Politècnica de Catalunya. Departament d’Enginyeria Química. Grup de Biotecnologia Molecular i Industrial; Spain.Fil: Tzanov, Tzanko. Universitat Politècnica de Catalunya. Departament d’Enginyeria Química. Grup de Biotecnologia Molecular i Industrial; Spain.Fil: Pérez, Leonardo Martín. Pontificia Universidad Católica Argentina. Facultad de Química e Ingeniería del Rosario. Instituto de Investigaciones en Ingeniería Ambiental, Química y Biotecnología Aplicada (INGEBIO); Argentina.Fil: Pérez, Leonardo Martín. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina.