info:eu-repo/semantics/article
Hepatitis C virus genotypes in Cordoba, Argentina. Unexpected high prevalence of genotype 2
Autor
Re, Viviana Elizabeth
Lampe, Elisabeth
Yoshida, Clara Fumiko
De Oliveira, Jaqueline Mendes
Lewis Ximenez, Lia
Spinsanti, Lorena
Elbarcha, Osvaldo César
Contigiani, Marta Silvia
Institución
Resumen
To determine hepatitis C virus (HCV) genotypes circulating in the central region of Argentina, 96 consecutive anti-HCV positive subjects were studied. The presence of HCV RNA was detected in 60 samples by RT-nested PCR of the 5′ noncoding region (5′ NCR). Genotyping was performed by restriction fragment length polymorphism analysis of 5′ NCR region combined with PCR using type-specific primers of the core region. The groups of individuals in this study included hemophilia and hemodialysis patients, injecting drug users, screened blood donors, and patients with acute or chronic liver disease, all from Córdoba, Argentina. Overall, genotype 2 was the most prevalent (55.0%), followed by genotypes 1 (38.3%), and 3 (5.0%). Within genotype 1, subtype 1b was the most prevalent. An unexpected high prevalence of genotype 2 (61.9%) was found among patients with acute or chronic HCV infection (without known risk factors). These figures differ from other cohorts from East-Argentina where genotype 1 has been found as the most prevalent. This indicates that regional differences of genotype distribution might exist between Central and East Argentina. Fil: Re, Viviana Elizabeth. Universidad Nacional de Córdoba. Facultad de Medicina; Argentina Fil: Lampe, Elisabeth. Fundação Oswaldo Cruz, Departamento de Virologia, Laboratorio de Hepatities Virais, Rio de Janeiro, Brazil Fil: Yoshida, Clara Fumiko. Fundação Oswaldo Cruz, Departamento de Virologia, Laboratorio de Hepatities Virais, Rio de Janeiro, Brazil Fil: De Oliveira, Jaqueline Mendes. Fundação Oswaldo Cruz, Departamento de Virologia, Laboratorio de Hepatities Virais, Rio de Janeiro, Brazil Fil: Lewis Ximenez, Lia. Fundação Oswaldo Cruz, Departamento de Virologia, Laboratorio de Hepatities Virais, Rio de Janeiro, Brazil Fil: Spinsanti, Lorena Ivana. Universidad Nacional de Córdoba. Facultad de Medicina; Argentina Fil: Elbarcha, Osvaldo César. Universidad Católica de Córdoba. Facultad de Ciencias Químicas; Argentina Fil: Contigiani, Marta Silvia. Universidad Nacional de Córdoba. Facultad de Medicina; Argentina