dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorRodrigues, Fabiana Aparecida
dc.creatorde Laia, Marcelo Luiz
dc.creatorZingaretti, Sonia Marli
dc.date2014-05-20T13:17:41Z
dc.date2016-10-25T16:39:05Z
dc.date2014-05-20T13:17:41Z
dc.date2016-10-25T16:39:05Z
dc.date2009-02-01
dc.date.accessioned2017-04-05T19:38:44Z
dc.date.available2017-04-05T19:38:44Z
dc.identifierPlant Science. Clare: Elsevier B.V., v. 176, n. 2, p. 286-302, 2009.
dc.identifier0168-9452
dc.identifierhttp://hdl.handle.net/11449/4064
dc.identifierhttp://acervodigital.unesp.br/handle/11449/4064
dc.identifier10.1016/j.plantsci.2008.11.007
dc.identifierWOS:000262566600016
dc.identifierhttp://dx.doi.org/10.1016/j.plantsci.2008.11.007
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/853470
dc.descriptionWater stress decreases plant productivity. To detect genes expressed under drought conditions, we performed a gene expression study using drought tolerant (SP83-5073) and sensitive (SP90-1638) sugarcane plants. Gene expression profiles were monitored by macroarray membranes containing 3575 cDNA clones from sugarcane leaf libraries, and the results were confirmed by real time PCR analysis. In the tolerant cultivar, we identified 165 genes that were expressed in response to water stress, in contrast with the sensitive cultivar, in which a higher number of genes (n = 432) were responsive to the stress treatment. The number of expressed genes in the sensitive cultivar increased with the severity of water deficit. In despite few genes had been differentially expressed in tolerant plants, 94% of them were up-regulated by stress, while 45% of the genes expressed in sensitive plants were down-regulated under water stress conditions. Comparing the gene expression profiles verified 91 common genes between both cultivars, the majority of which were up-regulated by water deficit. Genes were organized according to roles in cellular metabolism. Important stress-related pathways were repressed in sensitive plants. In both cultivars, we observed a great number of unknown genes, which will provide news insights in water deficit tolerance studies. (C) 2008 Elsevier B.V. All rights reserved.
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.languageeng
dc.publisherElsevier B.V.
dc.relationPlant Science
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectWater stress
dc.subjectTranscriptome
dc.subjectMacroarray
dc.subjectGene expression
dc.subjectSugarcane
dc.titleAnalysis of gene expression profiles under water stress in tolerant and sensitive sugarcane plants
dc.typeOtro


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