Selection and validation of reference genes for gene expression studies by reverse transcription quantitative PCR in Xanthomonas citri subsp citri during infection of Citrus sinensis

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorJacob, Tiago R.
dc.creatorLaia, Marcelo L.
dc.creatorFerro, Jesus A.
dc.creatorFerro, Maria I. T.
dc.date2014-05-20T13:17:41Z
dc.date2016-10-25T16:39:05Z
dc.date2014-05-20T13:17:41Z
dc.date2016-10-25T16:39:05Z
dc.date2011-06-01
dc.date.accessioned2017-04-05T19:38:44Z
dc.date.available2017-04-05T19:38:44Z
dc.identifierBiotechnology Letters. Dordrecht: Springer, v. 33, n. 6, p. 1177-1184, 2011.
dc.identifier0141-5492
dc.identifierhttp://hdl.handle.net/11449/4063
dc.identifierhttp://acervodigital.unesp.br/handle/11449/4063
dc.identifier10.1007/s10529-011-0552-5
dc.identifierWOS:000291655200015
dc.identifierhttp://dx.doi.org/10.1007/s10529-011-0552-5
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/853469
dc.descriptionXanthomonas citri subsp. citri (Xcc) causes citrus canker, a worldwide disease found mainly in sweet oranges (Citrus sinensis (L.) Osbeck). The expression of nine candidate internal reference genes was analyzed in Xcc grown alone and during C. sinensis infection to identify genes most suitable for comparative expression studies in Xcc using reverse transcription quantitative PCR (qRT-PCR). The stability of these genes was determined using the programs geNorm, NormFinder and BestKeeper. The genes most suitable for data normalization during C. sinensis infection were atpD, rpoB, gyrA and gyrB. The use of at least three reference genes is essential for accurate data normalization in Xcc.
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.languageeng
dc.publisherSpringer
dc.relationBiotechnology Letters
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectGene expression
dc.subjectqRT-PCR
dc.subjectReference genes
dc.subjectXanthomonas citri subsp. citri
dc.titleSelection and validation of reference genes for gene expression studies by reverse transcription quantitative PCR in Xanthomonas citri subsp citri during infection of Citrus sinensis
dc.typeOtro


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